Dr. Maisch Exsil 300 HAAX, 3 µm, 300 Å, 1 g bulk Material for HPLC Columns - 5136608.0001

Dr. Maisch Exsil 300 HAAX, 3 µm, 300 Å, 1 g bulk Material for HPLC Columns

Product Number: 5136608.0001

Exsil 300

• Alternative to Vydac TP phases
• Pore Size: 300 (+/-10) Å
• Pore Volume: 0.78 ml/g
• Surface Area: 100 m2/g
• Particle sizes: 3, 5 and 10 μm
• Phases: Silica, C4, C8, C18, PAH and HAAX
• Exsil 300: Columns with a pore size of 300 Å Angstroms (30 nm)

Exsil 300 HAAX

Exsil HAAX has been evaluated for the separation of carbohydrates and related compounds and found to give superior performance to conventional aminopropyl bonded phases.

Specifically it shows:

• Higher efficiencies
• Excellent selectivity
• Better stability to hydrolysis which allows the use of aggressive solvents
• Improved baseline response when used with the ELSD.

Exsil 300 HAAX (High Affinity Anion Exchanger) is a 300Ǻ spherical silica bonded with a polyamine coating. It was developed for anion exchange chromatography of proteins and its use in this context has been reported previously. In addition to providing good protein chromatography, the phase appeared to be robust and could withstand 30 column volumes of 0.1M sodium hydroxide before exhibiting deterioration.

As amine phases are used to separate carbohydrates, the HAAX phase was evaluated for this application. It was envisaged that the intrinsic stability might give the HAAX phase an advantage over conventional aminopropyl phases in terms of lifetime and a lower background on the ELSD.