World’s first ODS column with Anion and Cation Exchange. Low amount of strong ionic ligands, effective for strong ionic compounds.
Scherzo SW-C18 includes a few amount of strong ionic ligands which is a novel structural idea from
traditional ion-exchange columns. This ssurface structure offers strong ionic compounds elution.
Scherzo SW-C18 has a small amount of strong ionic ligands which is a novel structural idea from traditional ion-exchange columns. This surface structure provides better elution of strong ionic compounds siRNA (small interfering RNA), around 21mer base paired double stranded RNA, is getting lots of attention for next generation medicine. But it is very polar and has a lot of phosphoric acids inside, therefore difficult to analyze in RP mode.
SW-C18 can separate not only siRNA, but also ATP or oligo-nucleotides without adding an ionpairing reagent in the eluent. SW-C18 is applicable for strong ionic compounds like phosphoric compounds because there is a small amount of ionic ligands on the surface of the stationary phase.
Scherzo SW-C18 will provide excellent performance for basic drug compounds and ionic metabolites
between low and neutral pH conditions due to a small amount of strong anionic / cationic ligands.
SW-C18 may be useful for DMPK or metabolome studies.
Separation modes of Scherzo C18 columns
| Separation Mode | Stationary Phase | Properties |
| Reversed-Phase | Octadecyl | Increasing organic solvant composition (decreasing polarity of eluent) decreases retention. |
| Anion Exchange | Cation | Increasing iconic strength (salt or acid concentration decreases retention for acidic compounds. Generally low pH increases retention. |
| Cation Exchange | Anion | Increasing salt concentration decreases retention for basic compounds. SM-C18 retains more with increasing pH, while SS-C18 and SW-C18 retain more at lower pH. |
| Normal-Phase | Anion / Cation | Polar solutes, which cannot be retained with 100% aqueous eluent may be retained by using > 50% organic solvent composition due to electrostatic interation. |
Elution strategies by Scherzo C18 columns
| Non-ionic hydrophobic solutes |
Organic solvent composition should be optimized (similar to conventional ODS). Peak shape is often improved by using 0.1% acetic acid. |
| Ionic hydrophobic solutes |
A combination of organic solvent and 20-100mM of salt or acid at optimal pH should be used for separation of both acidic / basic compounds. |
| Weak ionic-polar solutes | SS-C18 is often the best choice for weak ionic polar solutes. Ionic strength should be increased for compounds that contain multiple ionic functional groups. Neutral pH conditions are required for mono-carboxylic acid compounds. |
| Strong ionic-polar solutes | SW-C18 may be useful when it is difficult to elute on SS-C18 or SM-C18. Strong ionic compounds have strong ionic interaction, so it is recommended to use multiple gradient elutions with ionic strength and organic solvent composition. |
