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Shimadzu Shim-pack HPC Columns

  • Shim-pack HPC columns have been specifically developed for the hydrophobic LC of biological high polymers such as proteins and nucleic acids.
  • The columns are packed with fully-porous spherical particles, 5μm in diameter and 300? in pore diameter. The effect of the residual silanol groups is minimized by the unique chemical treatment technique.
  • Ethyl groups or n-propyl groups are chemically bonded as stationary phase.

Hydrophobic LC and Reversed Phase LC

Both of these techniques use hydrophobic column packings, but are different in the following points.

  • The column packings for reversed phase LC are more hydrophobic than those for hydrophobic LC.
  •  In reversed phase LC, since organic solvent is generally used as mobile phase, the high-degree structures of proteins are often transmuted and the enzymes deactivated. In hydrophobic LC, on the other hand, since buffer solutions containing some salt are generally used, the high-degree structures of proteins are hardly transmuted.
  •  The retention of sample components is more dependent on the degree of hydrophobicity of the stationary phase in hydrophobic LC than in reversed phase LC. It is, therefore, more important to select the stationary phase, HPC-C2 or HPC-C3, in hydrophobic LC.


Item Name Product Image Price

Shimadzu Shimadzu Shim-pack HPC-C2, Ethyl LC Column 5 um, 4.0 mm x 5 cm - 228-17792-91

Shimadzu Shim-pack HPC-C2, Ethyl LC Column
Shimadzu Shimadzu Shim-pack HPC-C2, Ethyl LC Column 5 um, 4.0 mm x 5 cm - 228-17792-91
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Shimadzu Shimadzu Shim-pack HPC-C3, n-Propyl LC Column 5 um, 4.0 mm x 5 cm - 228-17793-91

Shimadzu Shim-pack HPC-C3, n-Propyl LC Column
Shimadzu Shimadzu Shim-pack HPC-C3, n-Propyl LC Column 5 um, 4.0 mm x 5 cm - 228-17793-91
Live chat below or contact us for latest price.
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