The ChromIQ operating software controls the Contichrom Discovery. It supports batch and multi-step connected batch processes and features tools for separation and purification with an intuitive, user-friendly interface.
ChromIQ has wizards for normal single column batch operation and for multi-step operation with up to three different columns. The wizards help to design batch single-step chromatography runs and automated two- or three-step batch runs. A set of methods are pre-optimized and built into the software (see below). The set can be amended by custom-made single- and multi-dimensional purification methods.
ChromIQ includes a number of features that are particularly helpful for automated processes, such as a buffer management system showing how many runs can be performed with the remaining buffer volumes, and a pressure sensor watchdog preventing the FPLC from running dry.
The software features include:
- Optimized standard methods for 1?, 2?, and 3?step processes
- Interactive process picture
- Single-click evaluation
- Easy data export (xlsx, csv, bmp, eps, emf)
- Pre-defined user groups with individual rights management
- Password protected user accounts
- Logging with time stamp and user name
- Electronic signature with check-sum of log and measurement files
If you are interested in a web-based live demo of the software, please contact us!
Walkthrough example: 2-step IMAC-SEC
Single- and multi-step processes can be set up easily with Discovery Wizard in the ChromIQ software. Below, the steps for setting up a 2-step purification for a His-tagged protein is shown. Relevant areas on the screenshots are marked in red.
The entry page shows options for 1-, 2- or 3-step purifications and a button to prepare the FPLC for storage. Click the “2-step Purification” button, which will turn blue.
The middle column now shows the different purification sequence options and also the possibility to run custom 2-step processes (yellow button). Click the “IMAC-SEC“ button which will turn blue. Now general information on the IMAC/SEC method is displayed on the right. Next, press “START 2-step”.
After entering the project and sample names, this screen with information for getting the FPLC ready to run is displayed. The picture shows where to mount which column (red highlights added for clarity). The “Mount Column” button shows detailed information and allows to flush the column with buffer for pre-equilibration.
The right table shows the required buffers for the selected purification method (red highlights added for clarity). Current and required buffer volumes are displayed. The table on the bottom left shows the connection between the color-coded buffer inlet tubes and the buffer tanks.
After all buffers have been filled and connected, the pumps can be purged to make sure that they contain the right buffers. The FPLC is then ready and the “START” button can be pressed.
The Software reminds you once more of the critical steps for preparing the purification run. Now you can “START the Run” to automatically purify your protein.