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Biomiga Blood Viral DNA/RNA Mini Kit
Product Number: VR6511-01
The EZgeneTM Viral DNA/RNA kit provides an easy and reliable method for isolating total viral DNA/RNA from plasma, serum, whole blood, urine and cell culture supernatant. This procedure has been tested for isolating nucleic acids from Hepatitis A, Hepatitis C and HIV. The isolated DNA/RNA can be used for PCR, RT-PCR and other downstream applications.
Storage and Stability
All other components can be stored at room temperature. All kit components are guaranteed for 1 year from the date of purchasing.
Before Starting
Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each steps.
Kit Contents
|
Catalog# |
VR6511-00 |
VR6511-01 |
VR6511-02 |
|
Preps |
4 |
50 |
250 |
|
Buffer PLY |
2.4 mL |
28 mL |
130 mL |
|
Wash Buffer * |
2 mL |
15 mL |
3 x 24 mL |
|
DEPC-Treated ddH2O |
500 µL |
10 mL |
50 mL |
|
ezBind Columns |
4 |
50 |
250 |
|
Collection Tubes |
8 |
100 |
500 |
|
User Menu |
1 |
1 |
1 |
*Add 8 mL (VR6511-00) or 60 mL (VR6511-01) or 96 mL (VR6511-02) 100% ethanol into each Wash Buffer bottle before use.
Trouble Shooting Guide
|
Problem |
Possible reason |
Suggested Improvement |
|
Low A260/A280 ratios |
Protein contamination |
Do a Phenol:Chloroform extraction. Loss of total RNA (up to 40%) should be expected. |
|
Low A260/A280 ratios |
Guanidine Thiocyanate contamination |
Add 2.5 volumes of ethanol and 0.1M NaCl (final concentration) to precipitate RNA. Incubate for 30 min at -20°C. Centrifuge at 13,000 rpm for 15 min at 4°C. Resuspend the RNA pellet in DEPC-treated water. |
|
Low Yield |
RNA in sample degraded |
Freeze samples immediately in liquid nitrogen and store at -70°C after collect it. |
|
Low Yield |
The binding capacity of the membrane in the spin column was exceeded |
Use of too much sample exceeding the binding capacity of spin column will cause the decreasing of total RNA yield. |
|
Low Yield |
Ethanol not added to buffer |
Add ethanol to the Wash Buffer. |
|
Genomic DNA contamination |
Too much total RNA sample was used in RT-PCR. |
Reduce total RNA amount used in RT-PCR to 50-100 ng. |
Important
Add 1% volume of β-mercaptoethanol to Buffer PLY before use and store at 4 oC.
Add 8 mL (VR6511-00) or 60 mL (VR6511-01) or 96 mL (VR6511-02) 100% ethanol into each Wash Buffer bottle before use.
Materials supplied by users
- Tabletop microcentrifuge
- 1.5 mL sterile tubes
- 100% ethanol
Note: Perform all steps including centrifugation at room temperature
- Model: VR6511-01
- Manufactured by: Biomiga
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