Description :

Introduction

Key to the kit is our proprietary DNA binding systems that allow the high efficient binding of DNA to our ezBind^TM matrix while proteins and other contaminates are removed under certain optimal conditions. Nucleic acids are easily eluted with sterile water or Elution Buffer. The purified DNA is ready for downstream applications such as cloning/subcloning, RFLP, sequencing, and transfection of HEK293 cells.

This kit is especially designed for purifying plasmid DNA from endA+ strains such as HB101, JM101, TG1 or their derived strains.

Important Notes

The yield of plasmid DNA depends on the origin of the replication and the size of the plasmid. The protocols are optimized for high copy number plasmid purification. For low copy number plasmids, both the culture volume and the buffer volume need to be scaled up 3 to 5 times. Please contact our customer service for further information and reference the table below for the commonly used plasmids,

Storage and Stability

Buffer A1 should be stored at 4 ℃ once RNase A is added. All other materials can be stored at room temperature. The Guaranteed shelf life is 18 months from the date of purchase.

Kit Contents

*Buffer N1 contains chaotropic salts, wear gloves and protective eyewear when handling.

**Add 60 mL (PD1711-01) or 96 mL (PD1711-02) 96-100% ethanol to DNA Wash Buffer before use. The Final ethanol is 80% (v/v)

Before Starting

Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each steps.

Important

• RNase A: Spin down RNase A vial briefly. Add the RNase A solution to Buffer A1 and mix well before use.
• Add absolute ethanol as instructed to DNA Wash Buffer before use.
• Buffer B1 precipitates below room temperature, it is critical to warm up the buffer at 37 ℃ to dissolve the precipitates before use.
• Keep the cap tightly closed for Buffer B1 after use.
• Ensure the availability of centrifuge capable of 13,000 rpm.
• Carry out all centrifugations at room temperature.
• Buffer N1 contains a chaotropic salt, wear gloves and protective eyewear when handling.

Materials supplied by users

• 96- 100% ethanol.
• 1.5 mL microcentrifuge tubes.
• High speed microcentrifuge.
• ddH₂O.