Description :

Introduction

The EZgene^TM Blood midi gDNA kit provides a fast and easy method for isolating gDNA from blood. The system utilizes the reversible nucleic acid-binding properties of Biomiga's ezBind membrane and the speed of spin column technology to yield high quality gDNA with the OD_260/280 ratio of 1.8-2.0. Up to 10 mL of fresh, frozen or anticoagulated whole blood treated with any common anticoagulant such as heparin, EDTA, or acid-citrate-dextrose can be readily processed at one time. This DNA Kit can also be used for the preparation of genomic DNA from buffy coat, serum, plasma, saliva, buccal swab and other body fluids. Purified DNA is ready for applications such as PCR, Southern Blotting, and Restriction Digestion.

The binding capacity per column is 500 μg of gDNA. Use less than10 mL of whole Blood or buffy coat is recommended.

Storage and Stability

All EZgene^TM Blood midi gDNA Kit components are guaranteed for at least 12 months from the date of purchase when stored as follows:

• Reconstituted Protease K store at -20°C.
• All other materials at room temperature (22-25°C).

Kit Contents

Before Starting

The kit is designed to be simple, fast, and reliable provided that all steps are followed diligently. Please read the entire booklet and get all necessary supplies and equipments.

Important

• Reconstitute Protease K as follows: Add 300 μL (GD2312-00), 1.5 mL (GD2312-01), or 3.8 mL (GD2312-02) Elution Buffer and vortex the vial briefly before use. Aliquot and store vials of reconstituted Protease K at -20°C.
• Dilute DNA Wash Buffer with absolute ethanol as follows:  Add 14 mL (GD2312-00) or 70 mL (GD2311-01) or 190 mL (GD2311-02) of absolute ethanol to each bottle. The final concentration is 70%.
• Under cool ambient conditions, precipitates may form in Buffer BL. In case of such an event, heat the bottle at 37°C to dissolve before use.
• Preheat the Elution Buffer at 70°C.

Materials provided by user

• Centrifuge capable of 5,000 x g
• Sterile 50 mL centrifuge tubes
• Water bath set to 55°C and 70°C
• Absolute ethanol
• PBS Buffer

Safety Information

Buffer BL contains chaotropic salts, which may form reactive compounds when combines with bleach, Do not add bleach or acidic solutions directly to the preparation waste, ware gloves and protective eyewear when handling.

Operating Protocol