Description :

Introduction

EZgene^TM Fungal gDNA Miniprep Kits allow rapid and reliable isolation of high-quality total cellular DNA from a wide variety of Fungal species and tissues. Up to 100 mg of wet tissue (or up to 50 mg dry tissue) can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of Biomiga's matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.

Overview

If using the EZgene^TM Fungal gDNA Miniprep Kit for the first time, please read this booklet to become familiar with the procedures before beginning. Dry or fresh fungal tissue is disrupted and then lysed in a specially formulated buffer containing detergent. Proteins, polysaccharides, and cellular debris are subsequently precipitated. Binding conditions are then adjusted and the sample is applied to a DNA spin-column. Two rapid wash steps remove trace contaminants such as residual polysaccharides, and pure DNA is eluted in water or low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

Storage and Stability

All components of the EZgene^TM Fungal gDNA Miniprep Kit are stable for at least 12 months from date of purchase when stored at 22℃-25℃. During shipment or storage in cool ambient conditions, precipitates may form in Buffer FG 3. It is possible to dissolve such deposits by warming the solution at 37℃.

Kit Contents

Before Starting

Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each steps.

Important:

•  Pre-heat Elution Buffer to 65^oC.
•  Dilute Wash Buffer Concentrate with ethanol as follows and store at room temperature. Add 8 mL (GD2416-00) or 60 mL (GD2416-01) or 96 mL (GD2416-02) absolute (96%-100%) ethanol to each bottle.
•  Choose the most appropriate protocol to follow. Procedures are described for each of dried and fresh (or frozen) specimens.

A. Dry Specimens   For processing ≦ 50 mg powdered tissue.  Yield is sufficient for several tracks on Southern assay.

B. Fresh or Frozen For processing ≦ 100 mg fresh (or frozen) powdered tissue. Yield is similar to A.

Trouble Shooting Guide

Limited Use and Warranty

This product is intended for in vitro research use only. Not for use in human. 

This product is warranted to perform as described in its labeling and in Biomiga's literature when used in accordance with instructions. No other warranties of any kind, express or implied, including, without limitation, implied warranties of merchantability or fitness for a particular purpose, are provided by Biomiga. Biomiga's sole obligation and purchaser's exclusive remedy for breach of this warranty shall be, at the option of Biomiga, to replace the products, Biomiga shall have no liability for any direct, indirect, consequential, or incidental damage arising out of the use, the results of use, or the inability to use it product.

For technical support or learn more product information, please contact us at (858) 603-3219 or visit our website at http://www.biomiga.com/.

Operating Protocol