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Description : Introduction The EZgeneTM Blood total RNA kit provides an easy and fast method for isolating total RNA from white blood cells within 30 min. Only trace genomic DNA exists in the purified RNA, which can be eliminated by DNase I treatment (See detail in the protocol) when it is necessary. Storage and Stability DNase I (optional) should be stored at -20 ℃. All other components can be stored at room temperature. All kit components are guaranteed for 12 months from the date of purchasing. Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each steps. Note: Perform all steps including centrifugation at room temperature Problem Possible reason Suggested Improvement Low A260/A280 ratios Protein contamination Do a Phenol: Chloroform extraction. Loss of total RNA (up to 40%) should be expected. Low A260/A280 ratios Guanidine Thiocyanate contamination Add 2.5 volumes of ethanol and 0.1M NaCl (final concentration) to precipitate RNA. Incubate for 30 min at -20 ℃. Centrifuge at 10,000 g for 15 min at 4 ℃ Resuspend the RNA pellet in DEPC-treated water. Low Yield RNA in sample degraded Freeze samples immediately in liquid nitrogen and store at -70 ℃ after collect it. Low Yield The binding capacity of the membrane in the spin column was exceeded Use of too much tissue sample exceeding the binding capacity of spin column will cause the decreasing of total RNA yield. Low Yield Ethanol not added to buffer Add ethanol to the RNA Wash Buffer and DNase Stop Buffer before purification. Genomic DNA contamination Too much total RNA sample was used in RT-PCR. Reduce total RNA amount used in RT-PCR to 50-100 ng. Genomic DNA contamination The sample may contain too much genomic DNA. Reduce the amount of starting tissue in the preparation of the homogenate. Most tissues will not show a genomic DNA contamination problem at 30 mg or less per prep. Reduce cell numbers to 1-2x106 or increase buffer volume and do multiple loadings to column. Catalog # Product Name Preps Price $ R6311-01 Tissue RNA kit 50 150.00 R6311-02 Tissue RNA kit 250 650.00 R6312-01 Tissue RNA midi kit 10 80.00 R6312-02 Tissue RNA midi kit 20 160.00 R6314-01 Tissue RNA maxi kit 10 120.00 R6314-02 Tissue RNA maxi kit 25 270.00 R6315-01 Tissue RNA mega kit 2 120.00 R6315-01 Tissue RNA mega kit 10 580.00 R6811-01 96-well tissue RNA kit 4x96 780.00 R6811-02 96-well tissue RNA kit 20x96 3300.00 R6411-01 Blood RNA mini kit 50 150.00 R6411-02 Blood RNA mini kit 250 680.00 R6412-01 Blood RNA midi kit 10 80.00 R6412-02 Blood RNA midi kit 25 180.00 R6414-01 Blood RNA maxi kit 10 120.00 R6414-02 Blood RNA maxi kit 25 270.00 R6812-01 96-well blood RNA kit 4x96 780.00 R6812-02 96-well blood RNA kit 20x96 3500.00Before Starting
Important
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Related EZgeneTM Products
- Model: R6414-01
- Manufactured by: Biomiga
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