Description :

Introduction

The EZgene^TM Viral RNA Kit provides an easy and reliable method for isolating total viral RNA from plasma or serum while enzyme inhibitors and other contaminates completely removed. This procedure has been tested for isolating nucleic acids from Hepatitis A, Hepatitis B, Hepatitis C and HIV. This kit can also be used to isolate viral DNA/RNA from urine and cell culture supernatant.

Storage and Stability

All other components can be stored at room temperature. All kit components are guaranteed for 12 months from the date of purchasing.

Kit Contents

Before Starting

Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each steps.

Important

•  Determine the volume of Buffer LY to be used and add 20 µL of β-mercaptoethanol (β-ME) per 1 mL Buffer LY before use. Buffer LY contains β-ME can be stored at room temperature for up to 1 month.
•  Crystals may form in Buffer LY, dissolve the precipitates at 37 ^oC before use.
•  Add 8 mL (R6620-00), 80 mL (R6620-01) or 3 x 96 mL (R6620-02) 100% ethanol to RNA Wash Buffer before use. The ethanol is 80%.

Materials supplied by users

•  Tabletop microcentrifuge and 1.5 mL sterile tubes.
•  Vacuum manifold if use vacuum protocol.
•  100% ethanol.

Note: Perform all steps including centrifugation at room temperature

Trouble Shooting Guide

Operating  Protocol :