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CLEARANCE Agilent Certified Clear Headspace Vials, Crimp, Flat-bottom, 20 mL, 100/Pk - 5182-0837
£44.10 £21.00
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CLEARANCE Glass Expansion Thermo iCAP 6000, Gold - 70-900-4002G, 5000-111
£201.03 £148.00
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CLEARANCE Thermo Scientific Chromacol 10 ml Crimp Top Headspace Vials, Clear, 10-CV, 125/Pk - CC-10-CV
£40.89 £31.15
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CLEARANCE Thermo Scientific CELL DISP ACRYLIC 300-1100NM 10 mm - 331720-000
£99.75 £76.00
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CLEARANCE Waters Protein-Pak Hi Res Q Ion-Exchange HPLC Column, 5 um, 4.6 mm ID x 100 mm Length, 1/pk - 186004931
£1,893.21 £1,190.70
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PROTEIN KW / PROTEIN LW columns separation by size also for high molecular weight
PROTEIN LW-803 HPLC Standard column - For antibody drugs analysis - Gel Filtration (GFC) - 3μm - 8.0mm I.D. x 300mm Length
Shodex Product Number: F6989303
The silica-based SEC (GFC) column for proteins and enzymes
Specifications:
| Description | PROTEIN LW-803 300x8mm |
|---|---|
| Chemistry Producers Term | LW |
| Gel material | Silica |
| Mode of Separation | Gel Filtration (GFC) / SEC Size Exclusion |
| Functional group | Hydrophilic polymer / Hydroxyl |
| Plates per column | ≥ 12,000 |
| Housing material | steel |
| Maximum pressure | 15 MPa (150 bar) |
| Usual flow rate | 1.0 mL/min |
| Maximum flow rate | 1.5 mL/min |
| Temperature range | 4 to 45°C |
| pH range | 3.0 to 7.5 |
| Salt concentration | ≤ 0.3 M |
| Shipping solvent | H2O |
| MW range | 10,000 - 700,000 |
| Exclusion limit | (1,000,000) |
| Internal Diameter | 8 mm |
| Length | 300 mm |
| Brand | PROTEIN |
| Particle Size | 3 µm |
| Pore Size | 300 Å (max. 1,000 Å) |
PROTEIN LW-803 and LW-403 4D Features
More and more monoclonal antibodies (mAb) are used for therapeutic drugs. The pore size of the Shodex PROTEIN LW-803 and LW-403 4D is suitable for this analysis.
- Pore size specifically controlled for analyzing proteins with a molecular weight of several hundred of thousand
- High performance analysis of antibody drugs and various proteins
- High lot-to-lot reproducibility
- Fulfills USP-NF L20 - Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5-10 μm in diameter, or a monolithic silica rod.
- Fulfills USP-NF L33 - Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 Da. It is spherical, silica-based, and processed to provide pH stability.
- Fulfills USP-NF L59 - Packing for the size-exclusion separations of proteins (separation by molecular weight) over the range of 5 to 7000 kDa. The packing is a spherical 1.5- to 10-μm, silica or hybrid packing with a hydrophilic coating.
PROTEIN LW-803 Possible Applications
- Calibration Curve for LW-803 (Proteins)
- Analysis of Epoetin According to USP-NF Method (LW-803)
- Analysis of Erythropoietin According to EP Method (LW-803)
- Analysis of Somatropin According to EP Method (LW-803)
- Comparison of SEC Separation of IgG between LW-403 4D and LW-803
- Comparison of SEC Separation of Standard Proteins between LW-403 4D and LW-803
- Monitoring Papain Digestion of Humanized Monoclonal IgG (LW-803)
- SEC Analysis of Antibody Drug Conjugate (LW-803)
- SEC Analysis of Rituximab (LW-803)
- Comparison of SEC Separation of IgG between LW-403 4D and LW-803
- Comparison of SEC Separation of Standard Proteins between LW-403 4D and LW-803
Range of Aqueous SEC (GFC) Columns: Silica-based Columns
| Product Code | Product Name | Plate Number (TP/column) |
Particle Size (μm) |
Pore Size (Å) |
Column Size (mm) I.D. x Length |
Shipping Solvent |
| KW-800 Series | Standard columns | Base Material: Silica | ||||||
| F6989000 | PROTEIN KW-802.5 | ≥ 21,000 | 5 | 400 | 8.0 x 300 | H2O |
| F6989103 | PROTEIN KW-803 | ≥ 21,000 | 5 | 1,000 | 8.0 x 300 | H2O |
| F6989104 | PROTEIN KW-804 | ≥ 16,000 | 7 | 1,500 | 8.0 x 300 | H2O |
| F6700131 | PROTEIN KW-G 6B | (guard column) | 7 | — | 6.0 x 50 | H2O |
| KW400 Series | High performance semi-micro columns | Base Material: Silica | ||||||
| F6989201 | KW402.5-4F | ≥ 35,000 | 3 | 400 | 4.6 x 300 | H2O |
| F6989202 | KW403-4F | ≥ 35,000 | 3 | 800 | 4.6 x 300 | H2O |
| F6989203 | KW404-4F | ≥ 25,000 | 5 | 1,500 | 4.6 x 300 | H2O |
| F6989204 | KW405-4F | ≥ 25,000 | 5 | 2,000 | 4.6 x 300 | H2O |
| F6700132 | KW400G-4A | (guard column) | 5 | — | 4.6 x 10 | H2O |
| For antibody drugs analysis | Standard columns | Base Material: Silica | ||||||
| F6989303 | PROTEIN LW-803 | ≥ 12,000 | 3 | 1,000 | 8.0 x 300 | H2O |
| F6700133 | PROTEIN LW-G 6B | (guard column) | 3 | — | 6.0 x 50 | H2O |
| For antibody drugs analysis | Semi-micro columns | Base Material: Silica | ||||||
| F6989403 | PROTEIN LW-403 4D | ≥ 11,000 | 1.9 | 1,000 | 4.6 x 150 | H2O |
| F6700134 | PROTEIN LS-G 4J | (guard column) | 1.9 | — | 4.6 x 20 | H2O |
- Model: F6989303
- Manufactured by: Showa Denko
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