Waters ACQUITY UPLC Refractive Index (RI) Detector

ACQUITY UPLC Refractive Index (RI) Detector

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Universal detection for your non-UV analytes

Improving resolution and throughput for your isocratic applications, such as advanced polymer chromatography (APC), food QC applications, pharmaceutical USP methods, and industrial process monitoring, is a priority.

The ACQUITY UPLC Refractive Index (RI) Detector offers unparalleled performance for the isocratic UHPLC/UPLC analysis of analytes without a UV chromophore. Its low-dispersion fluidics are designed to match the performance needed for narrow UPLC peaks while still delivering stable baseline performance, low noise, and a wide, linear dynamic range.

Overview

• Carry out universal detection of analytes without a UV chromophore that are separated by isocratic analysis
• Benefit from complete compatibility with a wide range of challenging solvents
• Achieve low-dispersion characteristics for the narrow peaks produced by ACQUITY UPLC PLUS series and ACQUITY APC systems
• Obtain low baseline drift due to a thermally isolated optics bench and highly efficient temperature equilibration of the incoming eluent

Recommended Use: For achieving unparalleled performance of isocratic UPLC or APC analysis of samples without a UV chromophore.

The ACQUITY UPLC H-Class System with the ACQUITY UPLC RI Detector.

Get faster answers for your quality control applications

Laboratories around the world have deployed UPLC technology to improve data quality and throughput by reducing analysis run times and increasing chromatographic resolution, making integration and quantification easier and more reliable. With the ACQUITY UPLC Refractive Index Detector, these same benefits can now be realized for samples with analytes that require RI detection due to the absence of a UV-chromophore.

Analysis of the five common food sugars in various matrices on the ACQUITY UPLC H-Class System with the ACQUITY UPLC RI Detector in under four minutes.

Low dispersion detection – a key component for APC

The ACQUITY APC System defines a new category of chromatographic polymer analysis that gives you more information about your polymers, faster than ever before. Combining low-dispersion system fluidics with rigid, small particle columns of a wide range of pore sizes, the system defines the ultimate in polymer peak resolution, particularly for low molecular weight oligomers.

The same 103 to 105 polystyrene standards were run on an ACQUITY APC System with a standard HPLC refractive index detector [A], and the ACQUITY UPLC RI Detector [B]. The loss in resolution due to the HPLC detector volume is clearly evident when zoomed in to the 103 standard. When the HPLC detector is used, all the resolution of the low MW oligomers is lost to the HPLC detector’s dispersion.

Simplify method transfer and uplevel your HPLC/RI methods

The benefits of transferring your existing HPLC methods to UPLC/UHPLC have been demonstrated time and time again, across multiple application types. Now, HPLC methods that require refractive index detection can also be seamlessly transferred to UPLC. Intuitive tools, including the ACQUITY UPLC Columns Calculator and the Reversed-Phase Column Selectivity Chart, are available to help you select the right UPLC stationary phase and scale your existing HPLC/RI methods to your UPLC/RI system efficiently and easily.

The transfer of the HPLC USP method for Topiramate to the ACQUITY UPLC H-Class System with the ACQUITY UPLC RI Detector has resulted in an 80% reduction in runtime while maintaining equivalent chromatographic performance.

Innovative technology for the chromatographic laboratory

The ACQUITY UPLC RI Detector’s innovative flow cell design addresses challenges of technical and environmental hurdles found when running an RI analysis. Coupled with a high energy LED, which delivers exceptional energy transmittance through the flow cell, the detector delivers maximum sensitivity while maintaining excellent peak shape. The integrated solvent valves provide automatic reference cell purging capabilities to maintain optimal baseline performance and solvent recycling, efficiently reducing costly solvent consumption. 

Sample flow enters the counter-current heat exchanger travelling through an internal capillary into the flow cell. When exiting the flow cell, it flows through a larger I.D. tubing that surrounds the inlet capillary. This thermally equilibrates the incoming sample-flow temperature, and dramatically minimizes drift.

Specifications: