Chromanik Sunrise C18-SAC HPLC Preparative Column, 120 A, 5 um, 250 mm Length x 10 mm ID - SA3781

ChromaNik Sunrise Octadecyl-SAC (C18-SAC) HPLC Preparative Column, 120Å, 5µm, 250mm Length x 10mm ID

Product Number: SA3781

Reversed-phase RP utilized silanol groups

Sunrise columns are very unique. Silanol Activity Control (SAC) technology leads not only adding an interaction by silanol groups but also a symmetrical peak forbasic compounds.

A long chain C28 phase shows high steric selectivity and larger retention for fat-soluble compounds than a C18 phase.

Both C18-SAC and C28 phase can achieve unique separation which a conventional C18 cannot do.

Regarding silanol group and tailing

In general, the silanol group remaining in the silica-based stationary phase in such as C18 (ODS) has been the cause of adsorption and peak tailing of a basic compound. Because silanol groups in proximity to the hydrophobic group is poorly hydrated, it indicates a high adsorption properties.

For reducing the effect of the residual silanol groups, a variety of end-capping techniques have been developed.

SAC (silanol activity control) technology

ChromaNik developed the SAC technology that only silanol groups with high absorption activity were selectively reduced and richly hydrated silanol groups without high absorption activity remained.

This technology allowed that a basic compound was separated without any peak tailing. Furthermore, not only hydrophobic interaction but also hydrogen bonding capacity and ion exchange interaction by richly hydrated silanol groups worked on reversed phase separation.

Sunrise C18-SAC - Silanol Activity Controlled C18 HPLC Column

Sunrise C18-SAC is bonded with octadecylsilane on a pure silica gel and controlled its silanol activity without end-capping. Its carbon content is 14%.

Sunrise C18 is the so-called fully end-capped C18 column. It shows the same separation behavior as a conventional C18 column. On the other hand, Sunrise C18-SAC shows hydrogen bond and ion-exchange interactions based on a residual silanol on the silica support in addition to reversed-phase separation. For example Sunrise C18 column separates a basic compound similarly as a conventional C18, while Sunrise C18-SAC makes retention of a basic compound be large because an ion-exchange interaction works although a non-ionic compound shows the almost same retention on both Sunrise C18 and C18-SAC. Furthermore, Sunrise C18-SAC shows large retention for a polar compound such as caffeine.

Separation on Sunrise C18-SAC is done including hydrogen bond and ion-exchange interaction based on silanol groups except for hydrophobic interaction. Control of pH and salt concentration of a mobile phase can regulate retention.

Specifications:

• Surface area: 340 m2 /g
• Pore volume: 1.0 mL/g
• Pore diameter: 12 nm (120 Å / 120A )
• pH range of C30 and C28: pH2-pH8, C18-SAC: pH2-pH7.5

Range of Sunrise Columns:

ChromaNik Comparison Table of HPLC and UHPLC Columns