Waters ACQUITY Premier HSS T3 Method Validation Kit with VanGuard FIT, 100A, 1.8 um, 2.1 x 150 mm, 3/pk - 186009638

Waters™ ACQUITY Premier HSS T3, 100 Å, 1.8 µm VanGuard FIT 2.1 x 150 mm MVK, 3/pk

Product Number: 186009638

Specifications:

The universal, silica-based bonded phase used for the ACQUITY HSS T3 sorbents is compatible with 100% aqueous mobile phase and should be your first choice when developing separations for polar and non-polar compounds. The ACQUITY Premier HSS T3 UPLC Columns exhibit ultra-low MS bleed and are fully scalable and transferable to HPLC separations. MaxPeak High Performance Surface hardware technology significantly reduces unwanted analyte/surface interactions that can lead to poor peak shape and losses in signal intensity. The VanGuard FIT Column design protects the column from contaminants and increases column lifetime.

HSS Method Validation Kits

The method validation kit (MVK) contains three columns each packed with a different lot of material. The universal, silica-based bonded phase used for the ACQUITY HSS T3 sorbents is compatible with 100% aqueous mobile phase and should be your first choice when developing separations for polar and non-polar compounds. The ACQUITY HSS T3 UPLC columns exhibit ultra-low MS bleed and are fully scalable and transferable to HPLC separations. MaxPeak High Performance Surface hardware technology significantly reduces unwanted analyte/surface interactions that can lead to poor peak shape and losses in signal intensity. The VanGuard Fit column design protects the column from contaminants and increases column lifetime.

UPLC Column Protection – VanGuard Pre-Columns

Contamination resulting from the analysis of samples present within complex matrices, or that are particulate-laden, may result in reduced column lifetime if not properlymaddressed. VanGuard™ Pre-Columns are ideally suited for the physical and chemical protection of ACQUITY UPLC Columns.

Directly compatible with UPLC pressures up to 18,000 psi [1241 bar], this ultra-low dispersion direct connect guard column is specifically engineered to preserve the lifetime of an ACQUITY UPLC Column without negatively impacting its separation performance.

ACQUITY Premier Columns

Stop looking and start seeing – no compromise required

Reduce the risks associated with unpredictable chromatography with ACQUITY Premier Columns with MaxPeak High Performance Surfaces (HPS) Technology, which allow you to see and do more by reducing analyte/surface interactions.

With Waters ACQUITY Premier Columns, chromatographers can harness the separation efficiency improvements and increased flow rates of ultra-performance liquid chromatography (UPLC) technology, improving analyte resolution, sensitivity, business productivity, and profitability. As the next evolution in the ACQUITY UPLC Column family, ACQUITY Premier Columns are built on the industry-leading sub-2-µm particle technologies from Waters you trust.

• Reduces column conditioning and passivation for higher productivity
• Increases reproducibility from analysis to analysis, for more confidence in analytical results
• Reduces non-specific absorption for increased sensitivity, improved peak shape, and easier analyte integration
• Provides superior separation speed, sensitivity, and resolution with best-in-class column efficiency
• 3 UPLC particles of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)
• Reversed-phase and HILIC stationary phases, including C₁₈, Embedded-Polar, and Amide
• Application-specific column chemistries for peptides, proteins, oligonucleotides, and glycan analysis
• Prolonged column lifetimes with VanGuard FIT Columns, and easy-to-use, all-in-one guard/column design

Recommended Use: For increased sensitivity, improved peak shape, and less column equilibration time in methods development.

High Strength Silica [HSS] Particle Technology

To complement Waters revolutionary Hybrid Particle Technology [HPT], a mechanically tolerant, silica-based material was designed to withstand UPLC pressures. High Strength Silica [HSS] particle technology was born from an innovative synthetic process that significantly increases the mechanical stability of silica while maintaining pore volumes similar to that of HPLC silica-based materials. T he result is a novel particle technology that provides increased retentivity compared to hybrid particles while serving as the ideal substrate to create stationary phases that provide alternate selectivity.

ACQUITY Premier HSS T3 Columns

The ACQUITY UPLC HSS T3 Column is ideally suited for the enhanced retention of polar compounds and metabolites by reversed-phase LC. This low-ligand density C18 column enables analytes to more readily access the pore structure of the material, providing balanced retention of polar and hydrophobic molecules without the need for ion-pair reagents.

• Ideal for the enhanced retention of polar and non-polar compounds
• Compatible with 100% aqueous mobile phases
• Mechanically stable at elevated pressures

ACQUITY UPLC columns

ACQUITY UPLC columns are the most technologically advanced LC columns ever created and are the only columns designed, tested and certified to withstand the pressures of UPLC Technology. Based upon the highly efficient 1.7 µm Bridged Ethyl Hybrid (BEH) particles and 1.8 µm High Strength Silica (HSS) particles, these columns are the first and only columns that allow separation scientists to achieve maximum speed, sensitivity and resolution without compromise. The particles and chemistries of the ACQUITY UPLC column family were carefully chosen to produce the ideal combination of ultra-performance efficiencies, wide pH range and complementary selectivities. ACQUITY UPLC BEH columns and ACQUITY UPLC HSS columns are the only columns recommended for use with ACQUITY UPLC Systems.

There is more to creating a UPLC particle than synthesizing a small particle. Many HPLC particles do not possess the mechanical stability and structural integrity to withstand UPLC operating pressures (e.g., 15000 psi/1000 bar). Why is pressure tolerance important? In order to realize the efficiency gains of sub-2 μm particles, the ability to routinely operate at higher linear velocities (e.g., higher flow rates) is required. These higher linear velocities combined with small, sub-2 μm particles result in higher operating backpressures. Waters has created two highly efficient, pressure-tolerant UPLC particles: the 1.7 μm Ethylene Bridged Hybrid (BEH) particle and the 1.8 μm High Strength Silica (HSS) particle.

The first ACQUITY UPLC particle created was the 1.7 μm Ethylene Bridged Hybrid (BEH) particle. This second generation hybrid particle is one of the key enablers behind UPLC technology and is available in five column chemistries: C18, C8, Shield RP18, Phenyl and HILIC. Because this is a hybrid particle, a wider usable pH range (up to pH 1-12) makes method development faster and easier. BEH particles are also available in HPLC particle sizes (2.5, 3.5, 5 and 10 μm) in the XBridge family of HPLC columns, thus allowing seamless transfer between HPLC and UPLC separations.

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