Waters ACQUITY UPLC HSS Cyano (CN) VanGuard Pre-column, 100A, 1.8 um, 2.1 mm X 5 mm, 3/pk - 186005995

Waters™ ACQUITY UPLC HSS Cyano (CN) VanGuard Pre-column, 100Å, 1.8 µm, 2.1 mm X 5 mm, 3/pk

Product Number: 186005995

Specifications:

ACQUITY UPLC HSS CN chemistry is an ultra-performance general purpose propyl cyano bonded phase that provides superior peak shape for bases, increased retention, and extremely long column lifetimes at low pH. ACQUITY HSS CN columns are fully scalable and transferable to HPLC separations.

The ACQUITY UPLC HSS Cyano Column offers an extended pH range of 2 to 8, enabling reliable separations across a wide range of acidic and alkaline conditions.

To get exceptional peak shape and reproducibility in low- to mid-pH conditions, you need UPLC technology and a column that’s more than up for the task. That’s where the ACQUITY UPLC HSS Cyano (CN) Column comes into consideration. Thanks to the general-purpose propyl-cyano bonded phase, this cyano column offers superior peak shape for bases and increased retention of polar compounds. That balance between polar- and non-polar functionalities is achieved because of this column’s unique chemistry. What’s more, this ACQUITY UPLC Column is fully scalable and transferable to HPLC separations. That kind of flexibility has been desired by scientists everywhere, and we’re happy to deliver with the ACQUITY UPLC HSS CN Column.

The ACQUITY UPLC HSS Cyano Column was built with method development scientists in mind, as it can help you easily develop robust methods that are compatible with all modern chromatographic detection modes. To add to that flexibility, this CN column also offers low hydrophobicity and unique selectivity when compared to straight-chain alkyl columns. And thanks to the High-Strength Silica base particle, this column is able to achieve ultra-stable retention under demanding conditions. You need only look at the ACQUITY UPLC HSS Cyano Column’s pH range to see that’s true.

All ACQUITY UPLC Columns are classified by their terrific separation power and efficiency, but what else do they offer? For one, ACQUITY UPLC separation technology can help chromatographers harness the increased flow rates of UPLC and improve analyte sensitivity all at once. With more than 50 column dimensions and configurations in the ACQUITY UPLC family, you’re sure to find the right match for your LC-MS analyses.

With superior separation performance comes other considerations, though. To keep the ACQUITY UPLC HSS Cyano Column performing at its best, you need to follow proper column cleaning and regeneration directions. But if you’re using various chemicals and solvents in the same column, particulate fouling can become a concern. That’s why we recommend equipping your analytical column with an ACQUITY UPLC HSS Cyano (CN) VanGuard Pre-column. The pre-column threads easily into your analytical column for a seamless column protection workflow.

UPLC Column Protection – VanGuard Pre-Columns

Contamination resulting from the analysis of samples present within complex matrices, or that are particulate-laden, may result in reduced column lifetime if not properly addressed. VanGuard™ Pre-Columns are ideally suited for the physical and chemical protection of ACQUITY UPLC Columns. Directly compatible with UPLC pressures up to 18,000 psi [1241 bar], this ultra-low dispersion direct connect guard column is specifically engineered to preserve the lifetime of an ACQUITY UPLC Column without negatively impacting its separation performance.

High Strength Silica [HSS] Particle Technology

To complement Waters revolutionary Hybrid Particle Technology [HPT], a mechanically tolerant, silica-based material was designed to withstand UPLC pressures. High Strength Silica [HSS] particle technology was born from an innovative synthetic process that significantly increases the mechanical stability of silica while maintaining pore volumes similar to that of HPLC silica-based materials. T he result is a novel particle technology that provides increased retentivity compared to hybrid particles while serving as the ideal substrate to create stationary phases that provide alternate selectivity.

As more separation scientists around the world realize the benefits of UPLC technology in their applications, Waters continues to provide additional UPLC particle and chemistry solutions to meet these demands. Waters material scientists developed a new High Strength Silica (HSS) particle with the high mechanical stability and appropriate morphology necessary to provide long column lifetimes and UPLC efficiencies at pressures up to 15000 psi (1000 bar). This 1.8 μm UPLC HSS particle is designed and tested specifically for use in UPLC separations.

ACQUITY UPLC columns

ACQUITY UPLC columns are the most technologically advanced LC columns ever created and are the only columns designed, tested and certified to withstand the pressures of UPLC Technology. Based upon the highly efficient 1.7 µm Bridged Ethyl Hybrid (BEH) particles and 1.8 µm High Strength Silica (HSS) particles, these columns are the first and only columns that allow separation scientists to achieve maximum speed, sensitivity and resolution without compromise. The particles and chemistries of the ACQUITY UPLC column family were carefully chosen to produce the ideal combination of ultra-performance efficiencies, wide pH range and complementary selectivities. ACQUITY UPLC BEH columns and ACQUITY UPLC HSS columns are the only columns recommended for use with ACQUITY UPLC Systems.

There is more to creating a UPLC particle than synthesizing a small particle. Many HPLC particles do not possess the mechanical stability and structural integrity to withstand UPLC operating pressures (e.g., 15000 psi/1000 bar). Why is pressure tolerance important? In order to realize the efficiency gains of sub-2 μm particles, the ability to routinely operate at higher linear velocities (e.g., higher flow rates) is required. These higher linear velocities combined with small, sub-2 μm particles result in higher operating backpressures. Waters has created two highly efficient, pressure-tolerant UPLC particles: the 1.7 μm Ethylene Bridged Hybrid (BEH) particle and the 1.8 μm High Strength Silica (HSS) particle.

The first ACQUITY UPLC particle created was the 1.7 μm Ethylene Bridged Hybrid (BEH) particle. This second generation hybrid particle is one of the key enablers behind UPLC technology and is available in five column chemistries: C18, C8, Shield RP18, Phenyl and HILIC. Because this is a hybrid particle, a wider usable pH range (up to pH 1-12) makes method development faster and easier. BEH particles are also available in HPLC particle sizes (2.5, 3.5, 5 and 10 μm) in the XBridge family of HPLC columns, thus allowing seamless transfer between HPLC and UPLC separations.

ACQUITY UPLC HSS column chemistries include HSS C18, HSS C18 SB (Selectivity for Bases) and T3. The HSS C18 chemistry is a fully endcapped, ultra-performance, general purpose C18 bonded phase that provides superior peak shape for bases, increased retentivity (vs. ACQUITY UPLC BEH C18 columns), and extremely long lifetimes under acidic conditions. The HSS C18 SB (Selectivity for Bases) chemistry is an unendcapped C18 bonded phase designed and optimized for low pH method development and offers alternate selectivities, especially for basic com- pounds, as compared to most modern, high coverage C18 chemistries. The HSS T3 chemistry is an aqueous mobile phase compatible C18 bonded phase that is designed to retain and separate small, water soluble, polar organic molecules, much like Atlantis T3 HPLC columns.

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