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HTA HT4000E SPE-LC Sample Prep Autosampler

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HT4000E AUTOMATED SPE-HPLC AUTOSAMPLER

HTA HT4000E: SPE-LC Sample Prep Workstation

SPE CARTRIDGES: Normal Phase, Reversed Phase, Ion Exchange, Affinity, Immunoaffinity SLE CARTRIDGES: Diatomaceous Earth Sorbent • FILTER CARTRIDGES

HT4000E is a flexible HPLC autosampler that can automate your Solid Phase Extraction routine. It takes care of sample purification up to the collection of eluted sample in vials (offline mode) or can proceed further up to the direct injection into the HPLC or LC-MS analyzer (online mode).

KEY FEATURES

Does more than any HPLC autosampler
Sample preparation accounts for up to 85% of the time taken for an analytical process. Therefore, it is important to select the correct instrumentation to fully maximize the process to give optimum results: HT4000E combining both SPE automation and HPLC autosampler capability is the prefect instrument to achieve your goals.

Robust Technology
Check the brochure and view the video below to learn more about workstation operations. In a very small bench space, HT4000E is able to provide automation up to 32 samples; for each sample the purification volume can be as large as 50ml. You can customize the sample racks to fit your applications and lab workflow.

Constant Flow Technology
The constant flow technology eliminates the SPE cartridge lot-to-lot reproducibility issue that is typical of systems based on constant pressure technology. Thanks to Constant Flow Technology, washing and elution are performed at a constant flow (no vacuum or gas pressure required), ensuring constant elution times for all the analyzed samples.

Easy SPE method design
HTAPREP software controls HT4000E: it gets users up to speed quickly and allows them to run samples immediately. Each sample can have a multiple-step methodology providing the flexibility required. The software uses an intuitive powerful drag and drop interface to allow the unit to control every individual aspect of the SPE process. This includes sample loading, washing, drying, flushing, elution, derivatization as well as HPLC/LC-MS injection

Not only SPE: Do more with your HT4000E!
HT4000E support a wide-range of clean-up procedures. Besides most common SPE cartridges (Normal, Reversed and Ion Exchange phases), HT4000E can be used for Affinity and Immunoaffinity cartridges as per Diatomaceous Earth Sorbent SLE cartridges. Furthermore HT4000E can be successfully used to automate sample filtration by the usage of filter cartridges, a substitute of the syringe filters.

 Configuration: 01Configuration: 02Configuration: 03
Syringe2.5 ml2.5 ml10 ml
Sample Capacity32 Samples32 Samples20 Samples
Primary sample vial4 ml8 ml20 ml
SPE cartridge size3 ml3 ml3 ml
Elution vial4 ml4 ml4 ml
Mixing Vial2 ml2 ml2 ml
SolventsFive nozzle and two 10ml vials
WasteInfinite waste
ValveValve HPLC F/SS/6-2/0.40mm Max Pressure: 40Mpa
Loop20 μL SS Loop

 

TECHNICAL SPECIFICATIONS

General features
Syringe volume:100, 250, 500 μL and 1, 2.5, 10 ml
Electrical control:LAN and TTL
Analytical performance 1
RSD:Full loop: ≤ 0.25% (typically 0.15%) Partial loop: ≤ 0.5%
Linearity (r):≥ 0.9999
Carry over 2:≤ 0.05% (with standard wash)
typically ≤ 0.01% (with int/ext needle wash)
Physical features
Dimensions (W x H x D):310 x 630 x 690 mm
Weight:15 kg
Power supply:100-240±10%Vac; 50-60Hz; 55W

1 For volume ≥0.5μL; the values provided are for 10-1000μL syringes
2 Tested with Uracile 100ppm in mobile phase 60% methanol : 40% water; Column C18; detector: UV fixed length

 

 


  • Model: HT4000E
  • Manufactured by: HTA