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HTA HT4000L Lab-Automation Ready LC Autosampler

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HT4000L: The LC Autosampler Lab-Automation Ready

Easy to use Large sample capacity Application versatility Modular and scalable Lab automation ready

For the same price of a conventional HPLC autosampler, you just get more; be ready to review your expectation about HPLC autosamplers! Enjoy the large sample capacity, the support for a wide range of sample vessels, the application versatility, the lab automation capabilities and the possibility to implement new modules and functionalities directly in your lab. Fits any brand HPLC and LC/MS.

KEY FEATURES

Large Sample Capacity and Wide Range of Sample Vessels

HT4000L can handle up to 176 2ml-vials per run or it can work with different kinds of sample vessels. Inject samples out of vials (2/4/6/10/20/40 ml), test tubes, tube blocks or plates including Vacutainer®, Eppendorf® tubes, and Well Format Tubes. Choose from standard racks or enquire for a custom design: custom racks pay for themselves in a few weeks. Furthermore, racks are easily interchangeable in order to allow quick switching across multiple configurations.

Application Versatility

HT4000L fits all liquid chromatography systems. It is able to look after the widest range of applications including analytical, prep, UHPLC, IC, nano, LC/MS… and many more!

Lab Automation Ready

HT4000L can work as a standard HPLC autosampler. However, you may want to delegate some sample preparation tasks: HT4000L can take care of them as no standard autosampler has been able to assist. Dilution, pre-column derivatisation, pH spiking and internal standard addition are available within the standard version. If standard sample preparation capabilities are not enough, get the HTAPREP option by which you can add support to complex and custom autosampler methods: endless possibilities, any sample preparation procedure or complex sample introduction become available.

Modular and Scalable

A wide range of accessories can be sourced and added in the field at any time; they are available to implement your autosampler with. They include: cooling module, vortex, vial heater and reaction cell, barcode reader (BCR), internal/external needle washing, flow cell, Mini-Uniprep® filter vials and more. We provide your LC autosampler at the best possible price; only at the time your needs change you will pay for the optional accessories to perform the new tasks without the hassle of buying a new autosampler. Your investment is future proof!

Enjoy your daily experience with HT4000L

Easy to use, easy to install and easy to service. We believe in a “robust by design” approach: HT4000L – based on cutting edge technologies and DFX design – well incarnates this philosophy making itself the ideal instrument for installation in any scenario. Set your method once using the complimentary HTA Autosampler Manager, then you just need to load samples and press START: experience a seamless integration with your Chromatography Data System.

UHPLC READY BIOCOMPATIBLE

UHPLC applications require an extended pressure range and a reduced amount of injected sample. HT4000L is optimised for such requirements and can be supplied with an UHPLC injection valve or can be upgraded in the field at a later time. Also in this configuration HT4000L features extraordinary analytical performance with the highest reliability.

To handle more complex samples and to assure covering a wider pH range, inert flow path valves are available. No need to compromise when it comes to running metal-sensitive samples or corrosive solvents/buffers. Furthermore, biocompatible applications can take advantage of the double wash step capability: as pre- and post-wash solvent in addition to A, B… F solvents you can also choose a combination of A+B, A+C…. F+E solvents for superior analytical performance (carryover adverse!).
LARGE-VOLUME CAPABILITY COOLING
The only real autosampler choice in the market for largevolume applications. We provide you with everything you need for your largevolume applications: large sample vessel support, large injections up to 10ml, large solvent capacity, large bore size injection valve (or injection port) for real and complete automation. The Cooling Module offers the capability to maintain samples at a controlled temperature. The cooling option upgrade can be performed in the field in a very short time: you don’t need to make a decision about cooling at the time the autosampler is sourced!

TECHNICAL SPECIFICATIONS

General features
Syringe volume: Standard: 100, 250, 500 μL and 1, 2.5, 10 μL
Nano-Volume Opt.: 5, 10, 25, 100 μL
Tray capacity (standard): 176 vials, 2ml (1 removable rack)
96 vials, 4ml (1 removable rack)
54 vials, 6/10/20ml (1 removable rack)
3 96-well plates (1 removable rack)
Custom trays available
Tray capacity (cooled) 121 vials, 2ml
42 vials, 6/10/20ml
2 96-well plates
Maintenance: Preventive counters available
Electrical control: LAN and TTL
Analytical Performance
RSD Full loop: ≤ 0.25% (typically 0.15%) Partial loop: ≤ 0.5%
Linearity (r): ≥ 0.9999
Carry over2: ≤ 0.05% (with standard wash) typically ≤ 0.01% (with int/ext needle wash)
Wash (Pre & Post Injection)
Washing cycle: up to 15 (Mode: every injection/sample/step)
Reagents: up to 2 different reagents for wash function
Stator wash: programmable
Filling
Sample volume: as low as step of 1ml μL(3)
Air volume: as low as step of 1ml μL(3)
Filling speed: 1-100μL/sec(3)
Viscosity delay: 0-25s
Bubble elimination: up to 15 pull up strokes
Diluition, derivatization, pH spiking, IS addiction
Sample dispatch: step of 1μL(3)
Reagent addiction: step of μL; up to 3 different reagents
Mixing cycle: programmable
Intermediate wash cycle: up to 15
Reaction time: 0-100 minutes (0.1 minutes step)
Physical features
Dimensions (WxHxD): 310 x 705 x 690 mm
Weight: 15kg
Power supply: 100-240±10%Vac; 50-60Hz; 55W
Cooling Module
Technology: Peltier
Temperature range conditioning: from 4°C to 40°C
Temperature accuracy: +/-1°C (typically +/-0.5°C) between the vials
Cooling capacity: about 20°C below the RT (on the vial)
Working ambient temperature: 25°C +/-10°C
Weight: about 5kg
Power supply: 100-240±10%Vac; 50-60Hz; 120W
Field installation: yes
Injection
Injection mode: Full Loop, Partial Loop
Flush Volume: programmable
Injection speed: 1-500μL/sec(3)
Analysis time: 0-999min
Wait time between consecutive injections: as low as 12sec(4)
Additional modules:
Among the most popular modules:
Barcode reader (BCR) • Flow Cell • Gripper for vial displacement • Internal/external needle washing • Sample Capacity Extension • Vial Heater/ Reaction Cell • Vortex

Free software “HTA Autosampler Manager (Standard Version)“ is provided for PC control; upgrade to “HTA Autosampler Manager (CFR 21 Part 11 Version)“ should be purchased separately. PC is required for setup, service, method and sequence editing.

HT4000L Operations

Learn about Software Integration

 


  • Model: HT4000L
  • Manufactured by: HTA