HT4000L: The LC Autosampler Lab-Automation Ready
• Easy to use • Large sample capacity • Application versatility • Modular and scalable • Lab automation ready
For the same price of a conventional HPLC autosampler, you just get more; be ready to review your expectation about HPLC autosamplers! Enjoy the large sample capacity, the support for a wide range of sample vessels, the application versatility, the lab automation capabilities and the possibility to implement new modules and functionalities directly in your lab. Fits any brand HPLC and LC/MS.
KEY FEATURES
Large Sample Capacity and Wide Range of Sample Vessels
HT4000L can handle up to 176 2ml-vials per run or it can work with different kinds of sample vessels. Inject samples out of vials (2/4/6/10/20/40 ml), test tubes, tube blocks or plates including Vacutainer®, Eppendorf® tubes, and Well Format Tubes. Choose from standard racks or enquire for a custom design: custom racks pay for themselves in a few weeks. Furthermore, racks are easily interchangeable in order to allow quick switching across multiple configurations.
Application Versatility
HT4000L fits all liquid chromatography systems. It is able to look after the widest range of applications including analytical, prep, UHPLC, IC, nano, LC/MS… and many more!
Lab Automation Ready
HT4000L can work as a standard HPLC autosampler. However, you may want to delegate some sample preparation tasks: HT4000L can take care of them as no standard autosampler has been able to assist. Dilution, pre-column derivatisation, pH spiking and internal standard addition are available within the standard version. If standard sample preparation capabilities are not enough, get the HTAPREP option by which you can add support to complex and custom autosampler methods: endless possibilities, any sample preparation procedure or complex sample introduction become available.
Modular and Scalable
A wide range of accessories can be sourced and added in the field at any time; they are available to implement your autosampler with. They include: cooling module, vortex, vial heater and reaction cell, barcode reader (BCR), internal/external needle washing, flow cell, Mini-Uniprep® filter vials and more. We provide your LC autosampler at the best possible price; only at the time your needs change you will pay for the optional accessories to perform the new tasks without the hassle of buying a new autosampler. Your investment is future proof!
Enjoy your daily experience with HT4000L
Easy to use, easy to install and easy to service. We believe in a “robust by design” approach: HT4000L – based on cutting edge technologies and DFX design – well incarnates this philosophy making itself the ideal instrument for installation in any scenario. Set your method once using the complimentary HTA Autosampler Manager, then you just need to load samples and press START: experience a seamless integration with your Chromatography Data System.
UHPLC READY | BIOCOMPATIBLE |
UHPLC applications require an extended pressure range and a reduced amount of injected sample. HT4000L is optimised for such requirements and can be supplied with an UHPLC injection valve or can be upgraded in the field at a later time. Also in this configuration HT4000L features extraordinary analytical performance with the highest reliability. |
To handle more complex samples and to assure covering a wider pH range, inert flow path valves are available. No need to compromise when it comes to running metal-sensitive samples or corrosive solvents/buffers. Furthermore, biocompatible applications can take advantage of the double wash step capability: as pre- and post-wash solvent in addition to A, B… F solvents you can also choose a combination of A+B, A+C…. F+E solvents for superior analytical performance (carryover adverse!). |
LARGE-VOLUME CAPABILITY | COOLING |
The only real autosampler choice in the market for largevolume applications. We provide you with everything you need for your largevolume applications: large sample vessel support, large injections up to 10ml, large solvent capacity, large bore size injection valve (or injection port) for real and complete automation. | The Cooling Module offers the capability to maintain samples at a controlled temperature. The cooling option upgrade can be performed in the field in a very short time: you don’t need to make a decision about cooling at the time the autosampler is sourced! |
TECHNICAL SPECIFICATIONS
General features | |
Syringe volume: | Standard: 100, 250, 500 μL and 1, 2.5, 10 μL |
Nano-Volume Opt.: 5, 10, 25, 100 μL | |
Tray capacity (standard): | 176 vials, 2ml (1 removable rack) |
96 vials, 4ml (1 removable rack) | |
54 vials, 6/10/20ml (1 removable rack) | |
3 96-well plates (1 removable rack) | |
Custom trays available | |
Tray capacity (cooled) | 121 vials, 2ml |
42 vials, 6/10/20ml | |
2 96-well plates | |
Maintenance: | Preventive counters available |
Electrical control: | LAN and TTL |
Analytical Performance | |
RSD | Full loop: ≤ 0.25% (typically 0.15%) Partial loop: ≤ 0.5% |
Linearity (r): | ≥ 0.9999 |
Carry over2: | ≤ 0.05% (with standard wash) typically ≤ 0.01% (with int/ext needle wash) |
Wash (Pre & Post Injection) | |
Washing cycle: | up to 15 (Mode: every injection/sample/step) |
Reagents: | up to 2 different reagents for wash function |
Stator wash: | programmable |
Filling | |
Sample volume: | as low as step of 1ml μL(3) |
Air volume: | as low as step of 1ml μL(3) |
Filling speed: | 1-100μL/sec(3) |
Viscosity delay: | 0-25s |
Bubble elimination: | up to 15 pull up strokes |
Diluition, derivatization, pH spiking, IS addiction | |
Sample dispatch: | step of 1μL(3) |
Reagent addiction: | step of μL; up to 3 different reagents |
Mixing cycle: | programmable |
Intermediate wash cycle: | up to 15 |
Reaction time: | 0-100 minutes (0.1 minutes step) |
Physical features | |
Dimensions (WxHxD): | 310 x 705 x 690 mm |
Weight: | 15kg |
Power supply: | 100-240±10%Vac; 50-60Hz; 55W |
Cooling Module | |
Technology: | Peltier |
Temperature range conditioning: | from 4°C to 40°C |
Temperature accuracy: | +/-1°C (typically +/-0.5°C) between the vials |
Cooling capacity: | about 20°C below the RT (on the vial) |
Working ambient temperature: | 25°C +/-10°C |
Weight: | about 5kg |
Power supply: | 100-240±10%Vac; 50-60Hz; 120W |
Field installation: | yes |
Injection | |
Injection mode: | Full Loop, Partial Loop |
Flush Volume: | programmable |
Injection speed: | 1-500μL/sec(3) |
Analysis time: | 0-999min |
Wait time between consecutive injections: | as low as 12sec(4) |
Additional modules: | |
Among the most popular modules: | |
Barcode reader (BCR) • Flow Cell • Gripper for vial displacement • Internal/external needle washing • Sample Capacity Extension • Vial Heater/ Reaction Cell • Vortex |
Free software “HTA Autosampler Manager (Standard Version)“ is provided for PC control; upgrade to “HTA Autosampler Manager (CFR 21 Part 11 Version)“ should be purchased separately. PC is required for setup, service, method and sequence editing.
HT4000L Operations
Learn about Software Integration
- Model: HT4000L
- Manufactured by: HTA