HTA HT4000E Automated SPE-HPLC Sample Prep Workstation

HT4000E SPE-HPLC Chromatography Sample Prep Workstation

SPE CARTRIDGES: Normal Phase, Reversed Phase, Ion Exchange, Affinity, Immunoaffinity SLE CARTRIDGES: Diatomaceous Earth Sorbent • FILTER CARTRIDGES

• Do more than any HPLC autosampler • Easy SPE method design • Robust cutting-edge technology • Constant flow elution technology • Improve reproducibility and throughput • Reduce costs and delays

WHY MOVE TO AUTOMATION?

There are a number of reasons why you should automate the sample prep procedures you currently run manually. Through automation you can:

• increase productivity by running 24/7;
• improve repeatability and traceability;
• lower operator’s health and safety risks by reducing exposure to hazardous chemicals and reduce crosslab CV by increasing method robustness and facilitating method transfer.

HT4000E is a flexible HPLC autosampler that can automate your Solid Phase Extraction routine. It is part of HTA’s HT4000 series, which includes lab-automation-ready HPLC autosamplers (HT4000L) and sample prep workstations (HT4000A). Designed to automate SPE-LC(MS) applications, it takes care of sample purification through Solid Phase Extraction up to the collection of eluted sample in vials (offline mode) or can proceed further up to the direct injection

into the HPLC or LC-MS analyzer (online mode). Therefore, HT4000E represents a valid alternative to the traditional HPLC autosampler, because it also includes the SPE functionality for only a little investment. Solid Phase Extraction consists of multiple steps that require precise volumes, flow rates, timing and more that are not easy to achieve manually. Therefore, automating SPE improves reproducibility and accuracy, reduces the costs and delays associated with re-runs and increases the overall throughput by running samples 24/7. Sample preparation accounts for up to 85% of the time taken for an analytical process. Therefore, it is important to select the correct instrumentation to fully maximize the process to give optimum results: HT4000E combining both SPE automation and HPLC autosampler capability is the prefect instrument to achieve your goals.

KEY FEATURES

Does more than any HPLC autosampler

Sample preparation accounts for up to 85% of the time taken for an analytical process. Therefore, it is important to select the correct instrumentation to fully maximize the process to give optimum results: HT4000E combining both SPE automation and HPLC autosampler capability is the prefect instrument to achieve your goals.

Robust Technology

Check the brochure and view the video below to learn more about workstation operations. In a very small bench space, HT4000E is able to provide automation up to 32 samples; for each sample the purification volume can be as large as 50ml. You can customize the sample racks to fit your applications and lab workflow.

Constant Flow Technology

The constant flow technology eliminates the SPE cartridge lot-to-lot reproducibility issue that is typical of systems based on constant pressure technology. Thanks to Constant Flow Technology, washing and elution are performed at a constant flow (no vacuum or gas pressure required), ensuring constant elution times for all the analyzed samples.

Easy SPE method design

HTAPREP software controls HT4000E: it gets users up to speed quickly and allows them to run samples immediately. Each sample can have a multiple-step methodology providing the flexibility required. The software uses an intuitive powerful drag and drop interface to allow the unit to control every individual aspect of the SPE process. This includes sample loading, washing, drying, flushing, elution, derivatization as well as HPLC/LC-MS injection.

Not only SPE: Do more with your HT4000E!

HT4000E support a wide-range of clean-up procedures. Besides most common SPE cartridges (Normal, Reversed and Ion Exchange phases), HT4000E can be used for Affinity and Immunoaffinity cartridges as per Diatomaceous Earth Sorbent SLE cartridges. Furthermore HT4000E can be successfully used to automate sample filtration by the usage of filter cartridges, a substitute of the syringe filters.

The Software

HTAPREP software controls HT4000E via LAN communication. It gets users up to speed quickly and allows you to start running samples on day 1. The software supplied uses an intuitive powerful drag and drop interface to allow the unit to control every individual aspect of the SPE process. This includes sample loading, washing, drying, flushing, elution, derivatization as well as full loop or partial loop injection. The flexibility of the HT4000E software enables the unit to perform a wide variety of applications such as the collection of several fractions of an elution or the re-injecting of a sample into a different SPE tube if required.

VARIOUS CONFIGURATIONS AVAILABLE

TECHNICAL SPECIFICATIONS

1For volume ≥0.5ml; the values provided are for 10-1000ml syringes 2Tested with Uracile 100ppm in mobile phase 60% methanol : 40% water; Column C18; detector: UV fixed length

Sample Purification Process

Sample Racks overview