Waters ACQUITY UPC2 System

ACQUITY™ UPC2® System

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The ease of reversed-phase meets the power of normal-phase

In reversed-phase chromatography, polar compounds are eluted first, causing routine and complex separation challenges. With convergence chromatography, however, polar compounds are retained and eluted last - providing the separation power of normal-phase liquid chromatography (LC) with the ease-of-use of reversed-phase LC.

Using green and non-toxic compressed liquid CO₂ as a primary mobile phase, the ACQUITY UPC2® System delivers the ability to precisely vary mobile phase strength, pressure, and temperature. The ability to fine-tune the resolving power and selectivity of the system, enables better control over the retention of analytes for separating, detecting, and quantifying structural analogs, isomers, and enantiomeric and diastereomeric mixtures - all compounds that are often a challenge to separate by any other means.

• The chromatographic principles and selectivity of normal-phase LC
• The ease of use and method development simplicity of reversed-phase LC
• The use of gradients across the widest polarity range
• Chiral and achiral separations in one system, with unequaled speed and confidence

Recommended Use: To solve routine and complex separations challenges that require unparalleled selectivity and ease-of-use.

Bringing ease of use to normal-phase LC

By providing the ability to precisely vary mobile phase strength, pressure, and temperature, the Waters ACQUITY UPC² System gives scientists the power to better control the retention of analytes for separating, detecting, and quantifying structural analogs, isomers, and enantiomeric and diastereomeric mixtures.

The world's first convergence chromatography system

Unique in its capabilities, the pioneering ACQUITY UPC² System delivers what the rest of the industry never could - a system, based on the principles of normal-phase LC, with the ease-of-use of reversed-phase LC, and purposefully built for analytical performance, placing convergence chromatography atop the list of separations tools in the kit of all analytical chemists.

Unparalleled resolving power and selectivity

With its separation potential for a far broader chemical spectrum of applications than that of reversed-phase LC-based instrumentation, the ACQUITY UPC² System leverages convergence chromatography to enable superior performance, using inexpensive, compressed CO₂ as a non-toxic mobile-phase.

Retention mechanisms and orthogonality to RPLC

Harnessing what's now known as convergence chromatography, an evolution of the traditional SFC technique, the ACQUITY UPC² System has the power to create selective separations of structurally similar chiral and achiral compounds.

The miscibility of CO₂ with a wide range of polar and non-polar organic solvents has made the liquid CO₂-based mobile phase versatile enough to separate a much wider range of compounds than reversed-phase LC, especially for mixtures containing polar compounds. Not only can CO₂-based solvents be used with both polar and non-polar stationary phases, but chromatography can be influenced by modulating solvent gradients with a much wider choice of columns using the same mass spectrometry-compatible co-solvents.

Being orthogonal to reversed-phase LC, UPC² separations often show an inverse order of elution among groups of analytes. In combination with various detection techniques, this orthogonality is valuable for confirming analyte identity in complex matrices.

Specifications: