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Superdex™ 75 / Superdex™ 200 Columns
- High-resolution separations of proteins, peptides, and other biomolecules according to size.
- Ideal for the polishing step in a purification procedure.
- Separations in two ranges from Mr 3000 up to 600 000 (globular proteins).
- Easy and predictable scale-up.
- Excellent reproducibility and durability.
- For rapid protein homogeneity analysis or purity check use the 5/150 GL short columns
Superdex™ 75 / Superdex™ 200 Columns
Technical Information
Superdex prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. High performance gel filtration offers fast separations with high resolution for a variety of applications including protein purification, studies of complex formation, and screening of uncharacterized samples.
Superdex 75 5/150 GL and Superdex 200 5/150 GL have small bed volumes, which is useful for a range of applications such as screening solubilization conditions for membrane proteins, the study of protein-protein interactions, and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.
Superdex columns 10/300 GL and 5/150 GL are prepacked Tricorn columns, see Tricorn™ Column Family, that provide excellent reproducibility and durability in purification using a chromatography system.
TECHNICAL SPECIFICATIONS Superdex™ Exclusion limit (Mr) Superdex™ 75 1 × 105 globular protein Superdex™ 200 1.3 × 106 globular protein Separation range (Mr) Superdex™ 75 3000-70 000 globular protein Superdex™ 200 10 000-600 000 globular protein Matrix Spherical composite of cross-linked agarose and dextran Average particle size 13 μm Chemical stability Stable in all common buffers:
1 M acetic acid, 8 M urea,
6 M guanidine hydrochloride,
30% isopropanol, 70% ethanol,
1 M NaOH (for cleaning in place) pH stability 3-12 (working and long term), 1-14 (short term) Storage 20% ethanol Storage temperature 4°C to 30°C Superdex™ 10/300 GL Columns (Tricorn)* Bed dimensions 10 × 300 mm Recommended sample volume 25-250 μl Bed volume 24 ml Max. pressure Superdex™ 75 18 bar (261 psi, 1.8 MPa) Superdex™ 200 15 bar (217 psi, 1.5 MPa) Max. flow rate (H2O at 25°C) Superdex™ 75 1.5 ml/min Superdex™ 200 1.0 ml/min Theoretical plates > 30 000 m-1 Superdex™ PC 3.2/30 Columns Bed dimensions 3.2 × 300 mm Bed volume 2.4 ml Recommended sample volume 2-25 μl Max. pressure Superdex™ 75 24 bar (348 psi, 2.4 MPa) Superdex™ 200 12 bar (174 psi, 1.2 MPa) Max. flow rate (H2O at 25°C) Superdex™ 75 0.100 ml/min Superdex™ 200 0.100 ml/min Theoretical Plates > 30 000 m-1 Superdex™ 5/150 column (Tricorn™) Bed volume 3 ml Bed dimensions 5 × 150 mm Recommended sample volume 4 - 50 μl Recommended flow rate 0.15 - 0.6 ml/min Max. pressure Superdex 75 1.8 MPa Superdex 200 1.5 MPa Max. flow rate (H20 at 25°C) Superdex 75 0.7 ml/min Superdex 200 0.8 ml/min Theoretical plates > 25000 m-1 * Columns are not suitable for use with ÄKTAprime™ plus system.
- Model: 17-1089-01
- Manufactured by: GE HealthCare
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