Waters ACQUITY UPLC BEH C18 Method Validation Kit, 130A, 1.7 um, 2.1 mm X 50 mm, 3/pk - 186004044

As the premier column for method development, the 1.7 um ACQUITY UPLC BEH C18 sorbent provides you with the most universal column choice for UPLC separations. The trifunctionally bonded BEH particle gives you the widest usable pH range (pH 1-12), superior low pH stability, and ultra-low column bleed for high sensitivity MS applications.

ACQUITY UPLC BEH Amide Columns

Since 2003, Waters has developed innovative stationary phases  for Hydrophilic Interaction Chromatography [HILIC] to overcome the  challenge of retaining and separating extremely polar compounds. Based on Waters novel ethylene bridged hybrid [BEH] particle technology, New ACQUITY UPLC® BEH Amide columns utilize a chemically stable, trifunctionally-bonded amide phase, enabling a new dimension in stability and versatility for HILIC separations.

Designed to retain polar analytes and metabolites that are too polar to retain by reversed-phase [RP] chromatography, ACQUITY UPLC BEH Amide columns facilitate the use of a wide range of mobile phase pH [2 -11] to facilitate the exceptional retention of polar analytes spanning a wide range in polarity, structural moiety and pKa.

In addition to enhanced retention of polar compounds, ACQUITY UPLC BEH Amide columns provide increased mass spectrometry response, direct compatibility with sample preparation eluates [PPT, LLE and SPE] as well as orthogonal selectivity compared to reversed-phase materials, making HILIC an attractive alternative separation technique.

ACQUITY UPLC columns

ACQUITY UPLC columns are the most technologically advanced LC columns ever created and are the only columns designed, tested and certified to withstand the pressures of UPLC Technology. Based upon the highly efficient 1.7 µm Bridged Ethyl Hybrid (BEH) particles and 1.8 µm High Strength Silica (HSS) particles, these columns are the first and only columns that allow separation scientists to achieve maximum speed, sensitivity and resolution without compromise. The particles and chemistries of the ACQUITY UPLC column family were carefully chosen to produce the ideal combination of ultra-performance efficiencies, wide pH range and complementary selectivities. ACQUITY UPLC BEH columns and ACQUITY UPLC HSS columns are the only columns recommended for use with ACQUITY UPLC Systems.

There is more to creating a UPLC particle than synthesizing a small particle. Many HPLC particles do not possess the mechanical stability and structural integrity to withstand UPLC operating pressures (e.g., 15000 psi/1000 bar). Why is pressure tolerance important? In order to realize the efficiency gains of sub-2 μm particles, the ability to routinely operate at higher linear velocities (e.g., higher flow rates) is required. These higher linear velocities combined with small, sub-2 μm particles result in higher operating backpressures. Waters has created two highly efficient, pressure-tolerant UPLC particles: the 1.7 μm Ethylene Bridged Hybrid (BEH) particle and the 1.8 μm High Strength Silica (HSS) particle.

The first ACQUITY UPLC particle created was the 1.7 μm Ethylene Bridged Hybrid (BEH) particle. This second generation hybrid particle is one of the key enablers behind UPLC technology and is available in five column chemistries: C18, C8, Shield RP18, Phenyl and HILIC. Because this is a hybrid particle, a wider usable pH range (up to pH 1-12) makes method development faster and easier. BEH particles are also available in HPLC particle sizes (2.5, 3.5, 5 and 10 μm) in the XBridge family of HPLC columns, thus allowing seamless transfer between HPLC and UPLC separations.

ACQUITY UPLC HSS column chemistries include HSS C18, HSS C18 SB (Selectivity for Bases) and T3. The HSS C18 chemistry is a fully endcapped, ultra-performance, general purpose C18 bonded phase that provides superior peak shape for bases, increased retentivity (vs. ACQUITY UPLC BEH C18 columns), and extremely long lifetimes under acidic conditions. The HSS C18 SB (Selectivity for Bases) chemistry is an unendcapped C18 bonded phase designed and optimized for low pH method development and offers alternate selectivities, especially for basic com- pounds, as compared to most modern, high coverage C18 chemistries. The HSS T3 chemistry is an aqueous mobile phase compatible C18 bonded phase that is designed to retain and separate small, water soluble, polar organic molecules, much like Atlantis T3 HPLC columns.

Related Products

• 186004475 - Oasis Method Development 96-well µElution Plate, 2 mg Sorbent per Well, 30 µm, 1/pk
• 186005837 - 96-well Sample Collection Plate, 700 µL Round well, 25/pk
• 186003975 - ACQUITY UPLC BEH C18 VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk
• 186006363 - Reversed-Phase QC Reference Material
• 186005666CV - TruView pH Control LCMS Certified Clear Glass, 12 x 32 mm, Screw Neck Vial, with Cap and preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk
• 186006360 - Neutrals QC Reference Material