Description :

Introduction

The EZgene^TM families of products are innovative systems that radically simplify extraction and purification of nucleic acids from a variety of sources. Key to the system is the new matrix that specifically, but reversibly, binds DNA or RNA under certain optimal conditions, allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or low salt buffer.

The 96-well Cycle Pure Kit is a convenient system for fast and reliable purification of up to 96 PCR products. The method uses ezBind technology to recover DNA bands 50 bp-40 kb free of oligo nucleotides, nucleotides, and polymerase in yields exceeding 80%. Binding conditions are adjusted by addition of a specially formulated buffer, and the sample is applied to a DNA Plate. Following a rapid wash step, DNA is eluted with deionized water (or low salt buffer) and ready for other applications. No organic extractions or alcohol precipitations means safe and rapid processing of multiple samples in parallel. The product is suitable for T-A ligations, PCR sequencing, restriction digestion, or various labeling reactions. In addition the kit can be used to purify DNA from any other enzymatic reaction.

Storage and Stability

All 96-well Cycle Pure Kit components are guaranteed for at least 24 months from the date of purchase when stored at 22-25℃. Under cool ambient conditions crystals may form in Buffer GC. Simply warm at 37 ℃ to dissolve.

Binding Capacity

Each well on the 96-well DNA Plate can bind 1~12 μg DNA.

Kit contents

* 2 mL 96-Deep Well Plate can be cleaned and reused. See page 7 for details.

Before Starting

Please read this booklet thoroughly to ensure that you are familiar with the entire procedure. 96-well Cycle Pure Kit is designed to be simple, fast, and reliable provided that all steps are followed diligently.

All steps must be performed at room temperature.

Operating protocal