Description :

Introduction

This fast and reliable kit is designed to recover DNA from random-primed or nick-translation labeling reactions. Salts, enzymes and free labels are effectively removed to yield pure DNA probes. Binding conditions are adjusted by addition of a specially formulated buffer that enables the binding of the DNA to the spin column. Following two rapid washing steps, DNA is eluted with deionized water or elution buffer and ready for other applications.

Storage and Stability

All components can be stored at room temperature. All kit components are guaranteed for 12 months from the date of purchasing.

Kit Contents

*Buffer GC contains chaotropic salts. Wear gloves and use protective eyeware when handling this solution. Buffer GC may form precipitates under cool ambient condition. Warm up the buffer at 37℃ to dissolve before use.

Before Starting

Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each steps.

Important

• DC3513-00 Add 8 mL absolute ethanol
• DC3513-01 Add 60 mL absolute ethanol
• DC3513-02 Add 96 mL absolute ethanol

Materials supplied by users

•  Tabletop microcentrifuge and 1.5 mL sterile tube
•  Vacuum manifold if use vacuum protocol
•  70% ethanol

Operating protocal