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Biomiga Gel Extraction Kit - DC3511-01

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Description :

Introduction

This fast and reliable kit is designed to recover DNA from agarose gels, and purify DNA fragments from PCR, RFLP, phosphorylation, labeling, ligation, hybridization and other enzymatic reactions. DNA fragments from 100 bp to 20 kb can be purified using the ezBindTM mini column with over 80-90 % recovery.

Storage and Stability

All components can be stored at room temperature. All kit components are guaranteed for 12 months from the date of purchasing.

Kit Contents

Catalog#

DC3511-00

DC3514-00

DC3511-01

DC3514-01

DC3511-02

DC3514-02

DC3511-03

DC3514-03

Preps

4

50

250

100

Buffer GC

3 mL

40 mL

200 mL

80 mL

DNA Wash Buffer*

2 mL

15 mL

3 x 24 mL

 2 x 15 mL

Elution Buffer

1 mL

10 mL

20 mL

15 mL

ezBind Mini Columns

4

50

250

100

User Manual

1

1

1

1

  

Safety Information

Buffer GC contains acidic acid and chaotropic salts, which may form reactive compounds when combines with bleach. Do not add bleach or acidic solutions directly to the preparation waste.

Before Starting

Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each steps.

Important

  •  Add 8 mL (DC3511/DC3514-00) or 60 mL(DC3511/DC3514-01) or 96 mL (DC3511/DC3514-02) or 60 mL (DC3511/DC3514-03) 100% ethanol to DNA Wash Buffer before use.
  •  A gel slice of 100 mg equals to a volume of 100 µL.
  •  Buffer GC may form precipitates under cool ambient condition. Warm up the buffer at 37°C to dissolve before use.
  •  Pre-warm aliquots of Elution Bufer or ddH2O at 55-60°C waterbath.

 Materials supplied by users

  •  Tabletop microcentrifuge and 1.5 mL microtubes.
  •  55-60°C waterbath.
  •  Vacuum manifold if use vacuum protocol.
  •  96~100 % ethanol.
  •  Isopropanol for DNA fragment less than 200 bp.

Perform all steps including centrifugation at room temperature!

Trouble Shooting Guide

Problems
Possible reasons
Suggested improvements

Low DNA yield

1.Not enough Buffer GC

2.Agarose gel doesn't melt completely

3. Reused electrophoresis buffer with increased pH.

4. Fragment < 200 bp

5. Fragment >10 kb

•1.Determine the volume of Buffer GC to be used correctly as instructed.

•2.Make sure to set the water bath to 55-60oC to allow gel to melt completely. Add more Buffer GC if necessary.

•3.Use fresh electrophoresis buffer.

•4.Add isopropanol as instructed.

•5.Incubate the column (after adding ddH20 or Elution Buffer) at 60oC for 15 min before elution.

No DNA yield

Forgot to add ethanol to DNA Wash Buffer

Add absolute ethanol to DNA Wash Buffer as instructed before use.

DNA sample floats out of well while loading agarose gel

Ethanol was not completely removed from the column following wash step

After the wash step, centrifuge the empty column with the lid open at top speed for 1-3 min. Repeat once.

Column clogged

Agarose gel doesn't melt completely

Make sure to melt the gel at 55-60oC before loading the sample to DNA column.

* FOR RESEARCH USE ONLY.

Operating protocal


  • Model: DC3511-01
  • Manufactured by: Biomiga

Additional Images