Description :

Introduction

The EZgene^TM Poly-Gel DNA Extraction Kit is designed for rapid and consistent recovery of ssDNA or dsDNA from acrylamide gels. Following PAGE, oligonucleotides are first visualized by UV shadowing or ethidium bromide staining and then cut out, soaked and eluted from using gel elution buffer. A specially formulated binding buffer is then added to the eluate to allow the binding of the DNA to the silica matrix. While proteins, salts, buffers, and other impurities are removed by wash buffer, the DNA is eluted sterile deionized water or low salt buffer. In addition, the spin column format allows parallel processing of multiple samples. The purified DNA is suitable for PCR, ligation reactions, hybridization techniques, or other procedures.

Storage and Stability

All components of the EZgene^TM  Poly-Gel DNA Extraction Kit are stable for at least 12 months from date of purchase when stored at 22-25°C. Under cool ambient conditions (such as occurs during shipping) a precipitate may form in buffers, simply warm up the solution to dissolve the precipitates.

Safety Information

* Buffer GC contains chaotropic salts that are irritants. Do not add bleach directly to the experiment waste. Take appropriate laboratory safety measures and wear gloves when handling.

Kit Contents

Before Starting

•  Water bath or incubator equilibrated to 65°C.
•  For isotopically labeled DNA, autoradiographic film
•  Microcentrifuge capable of 10,000 x g
•  Sterile 1.5 mL microcentrifuge tubes.
•  Dilute DNA Wash Buffer with absolute ethanol as follows

• DC3512-00 Add 8 mL absolute ethanol
• DC3512-01 Add 60 mL absolute ethanol
• DC3512-02 Add 96 mL absolute ethanol

Operating protocal