Description : The EZgeneTM Viral DNA/RNA kit provides an easy and reliable method for isolating total viral DNA/RNA from plasma, serum, whole blood, urine and cell culture supernatant. This procedure has been tested for isolating nucleic acids from Hepatitis A, Hepatitis C and HIV. The isolated DNA/RNA can be used for PCR, RT-PCR and other downstream applications. All other components can be stored at room temperature. All kit components are guaranteed for 1 year from the date of purchasing. Catalog# VR6511-00 VR6511-01 VR6511-02 Preps 4 50 250 Buffer PLY 2.4 mL 28 mL 130 mL Wash Buffer * 2 mL 15 mL 3 x 24 mL DEPC-Treated ddH2O 500 µL 10 mL 50 mL ezBind Columns 4 50 250 Collection Tubes 8 100 500 User Menu 1 1 1 *Add 8 mL (VR6511-00) or 60 mL (VR6511-01) or 96 mL (VR6511-02) 100% ethanol into each Wash Buffer bottle before use. Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each steps. Important Materials supplied by users Note: Perform all steps including centrifugation at room temperature Problem Possible reason Suggested Improvement Low A260/A280 ratios Protein contamination Do a Phenol:Chloroform extraction. Loss of total RNA (up to 40%) should be expected. Low A260/A280 ratios Guanidine Thiocyanate contamination Add 2.5 volumes of ethanol and 0.1M NaCl (final concentration) to precipitate RNA. Incubate for 30 min at -20°C. Centrifuge at 13,000 rpm for 15 min at 4°C. Resuspend the RNA pellet in DEPC-treated water. Low Yield RNA in sample degraded Freeze samples immediately in liquid nitrogen and store at -70°C after collect it. Low Yield The binding capacity of the membrane in the spin column was exceeded Use of too much sample exceeding the binding capacity of spin column will cause the decreasing of total RNA yield. Low Yield Ethanol not added to buffer Add ethanol to the Wash Buffer. Genomic DNA contamination Too much total RNA sample was used in RT-PCR. Reduce total RNA amount used in RT-PCR to 50-100 ng. Operating Protocol Introduction
Storage and Stability
Kit Contents
Before Starting
Trouble Shooting Guide
- Model: VR6511-02
- Manufactured by: Biomiga