Biomiga ezFilter 96-well Yeast Plasmid Kit - YD1291-02

Description :  

Introduction

The EZgene^TM lines of products are innovative system that radically simplifies the extraction of nucleic acids from a variety of sources. Key to the system is Biomiga's proprietary ezBind matrix that avidly, but reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or low salt buffer.

The 96-Well ezFilter Yeast Plasmid Isolation Kit is designed for rapid high-throughput purification of plasmid from yeast cultures grown and processed in a 96-well Plate format. The purification process may be performed by centrifugation or vacuum protocol. One 96-well plate can be processed manually within 60 minutes. The procedure has been developed and tested using a variety of yeast plasmids and low copy plasmids.

This protocol has been successfully used to isolate autonomous plasmids from S. cerevisiae. As a modified alkaline lysis procedure, genomic DNA is virtually eliminated from the preparation. Note that all centrifugation steps are to be performed at room temperature.

Storage and Stability

All 96-Well ezFilter Yeast Plasmid Kit components are guaranteed for at least 12 months from the date of purchase when stored as follows: The mixture of YP1/RNase A at 4 ℃; all other materials at 22-25 ℃. Store YP II tightly capped.

Kit Contents

* The 2 mL 96-Well Collection Plates are reusable.

Before Starting

Briefly examine this booklet and become familiar with each step. Prepare all components and have the necessary materials ready before starting.

Important

•  Add vial of RNase A to bottle of Buffer YP I and Store at 4℃
•  Dissolve the Lyticase to 150U/mL using SE Buffer
•  DNA Wash Buffer has to be diluted with absolute

Ethanol (~96-100%) as follows:

YD1291-S: Add 150 mL ~96%-100% ethanol

YD1291-01: Add 300mL ~96%-100% ethanol

YD1291-02: Add 325 mL ~96%-100% ethanol