Waters ACQUITY Premier SEC Protein Column with MaxPeak Premier SEC Guard and mAb Size Variant Standard, 250A, 1.7 um, 4.6 x 300 mm - 176004795

Waters™ ACQUITY Premier Protein SEC Column with MaxPeak Premier SEC Guard, 250Å, 1.7 µm, 4.6 x 300 mm Column, with mAb Size Variant Standard

Product Number: 176004795

Specifications

ACQUITY Premier Protein SEC 250Å, 1.7 µm Columns help scientists obtain reliable protein size variant separations (10,000 to approx 650,000 Daltons) via the synergistic coupling of Waters MaxPeak Premier High Performance Surfaces and novel BEH-PEO Particle technologies. These advancements in SEC column hardware and SEC particle technology work to minimize non-desired secondary ionic or hydrophobic interactions between proteins and the column to minimize costly method development work by allowing chromatographers to use a “generic” or “platform-type” method for many samples. Note:  The column includes, at no addtional cost, a vial of Waters mAb Size Variant Standard (p/n: 186009429)  It also includes the recommended MaxPeak Premier SEC 250Å, 2.5 µm, 4.6 x 30 mm Guard (p/n: 186009969) to help extend column life without sacrifycing desired component resolution. Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.  

MAXPEAK Premier Columns

HPLC method development is not without its challenges. What happens when your favorite column struggles to achieve your peak shape, sensitivity, or reproducibility goals?

Whether you’re developing or transferring methods, quickly achieve consistent results with MaxPeak Premier Columns. Available from sub-2 µm to 5 µm HPLC particle sizes, fully porous and solid-core, you can choose the column configuration that meets your needs and eliminate doubt from your chromatographic separations.

mAb Size Variant Standard

Waters mAb Size Variant Standard (p/n: 186009429) contains the NIST humanized monoclonal antibody (Reference Material 8671) and non-reduced IdeS digested NIST mAb fragments F(ab')2 (~100,000 Da) and (Fc/2)2 (~50,000 Da). By aliquoting small, standard amounts of IdeS fragments, Waters mAb size variant stardard can be effectiely used to test column and LC System ability to separate mAb aggregates, monomer, and fragments / clips via SEC.

ACQUITY Premier Columns

Stop looking and start seeing – no compromise required

Reduce the risks associated with unpredictable chromatography with ACQUITY Premier Columns with MaxPeak High Performance Surfaces (HPS) Technology, which allow you to see and do more by reducing analyte/surface interactions.

With Waters ACQUITY Premier Columns, chromatographers can harness the separation efficiency improvements and increased flow rates of ultra-performance liquid chromatography (UPLC) technology, improving analyte resolution, sensitivity, business productivity, and profitability. As the next evolution in the ACQUITY UPLC Column family, ACQUITY Premier Columns are built on the industry-leading sub-2-µm particle technologies from Waters you trust.

• Reduces column conditioning and passivation for higher productivity
• Increases reproducibility from analysis to analysis, for more confidence in analytical results
• Reduces non-specific absorption for increased sensitivity, improved peak shape, and easier analyte integration
• Provides superior separation speed, sensitivity, and resolution with best-in-class column efficiency
• 3 UPLC particles of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)
• Reversed-phase and HILIC stationary phases, including C₁₈, Embedded-Polar, and Amide
• Application-specific column chemistries for peptides, proteins, oligonucleotides, and glycan analysis
• Prolonged column lifetimes with VanGuard FIT Columns, and easy-to-use, all-in-one guard/column design

Recommended Use: For increased sensitivity, improved peak shape, and less column equilibration time in methods development.

ACQUITY Premier Protein SEC 250 Å Guards and Columns

• Sub-2-μm particle columns for UPLC separations
• 250 Å pore size
• High throughput & resolution SEC separations of biomolecules ranging from 10,000 to 650,000 Daltons on low dispersion UPLC Systems
• Available SEC Guard Columns to extend analytical SEC Column life
• Significantly reduce non-desired ionic and hydrophobic secondary interaction for desired SEC platform methods with MaxPeak Premier Technology and BEH-PEO SEC particle technology

SEC Columns

Size-based separations of peptides, proteins, and viral vectors

Get unmatched batch-to-batch reproducibility with the wide range of Waters Size-Exclusion Chromatography (SEC) Columns and standards for protein and peptide characterization.

SEC is a powerful technique for aggregate and fragment analysis in the research, development, and manufacturing of biotherapeutic proteins, such as insulin and monoclonal antibodies (mAb).?With SEC, also known as gel filtration, you can separate proteins based on their size (hydrodynamic radii versus absolute molecular weight) in solution, with larger sized species eluting before smaller proteins. 

The?pore size?and?volume of the SEC particles?used in the packed column drives this isocratic based technique, so the column you choose matters.?As the molecular weight of the proteins of interest increases, SEC columns with comparatively larger pore size particles are selected.

ACQUITY UPLC columns

ACQUITY UPLC columns are the most technologically advanced LC columns ever created and are the only columns designed, tested and certified to withstand the pressures of UPLC Technology. Based upon the highly efficient 1.7 µm Bridged Ethyl Hybrid (BEH) particles and 1.8 µm High Strength Silica (HSS) particles, these columns are the first and only columns that allow separation scientists to achieve maximum speed, sensitivity and resolution without compromise. The particles and chemistries of the ACQUITY UPLC column family were carefully chosen to produce the ideal combination of ultra-performance efficiencies, wide pH range and complementary selectivities. ACQUITY UPLC BEH columns and ACQUITY UPLC HSS columns are the only columns recommended for use with ACQUITY UPLC Systems.

There is more to creating a UPLC particle than synthesizing a small particle. Many HPLC particles do not possess the mechanical stability and structural integrity to withstand UPLC operating pressures (e.g., 15000 psi/1000 bar). Why is pressure tolerance important? In order to realize the efficiency gains of sub-2 μm particles, the ability to routinely operate at higher linear velocities (e.g., higher flow rates) is required. These higher linear velocities combined with small, sub-2 μm particles result in higher operating backpressures. Waters has created two highly efficient, pressure-tolerant UPLC particles: the 1.7 μm Ethylene Bridged Hybrid (BEH) particle and the 1.8 μm High Strength Silica (HSS) particle.

The first ACQUITY UPLC particle created was the 1.7 μm Ethylene Bridged Hybrid (BEH) particle. This second generation hybrid particle is one of the key enablers behind UPLC technology and is available in five column chemistries: C18, C8, Shield RP18, Phenyl and HILIC. Because this is a hybrid particle, a wider usable pH range (up to pH 1-12) makes method development faster and easier. BEH particles are also available in HPLC particle sizes (2.5, 3.5, 5 and 10 μm) in the XBridge family of HPLC columns, thus allowing seamless transfer between HPLC and UPLC separations.

Related Products

• MaxPeak Premier Protein SEC Guard, 250Å, 2.5 µm, 4.6 x 30 mm, 1/pk - 186009969
• mAb Size Variant Standard - 186009429
• wwPTFE Acrodisc Mini Syringe Filter, 0.45 μm 13 mm 100/Pk - 186009315
• ACQUITY Premier Protein SEC Column, 250Å, 1.7 µm, 4.6 x 150 mm with mAb Size Variant Standard plus Guard, 1/pk - 176004794
• ACQUITY Premier Protein SEC Column with MaxPeak Premier SEC Guard, 250Å, 1.7 µm, 4.6 x 150 mm, 1/pk - 176004783