Waters ACQUITY UPLC BEH C8 Column, 130A, 1.7 um, 2.1 mm X 100 mm, 3/pkg - 176000885

Waters™ ACQUITY UPLC BEH C8 Column, 130Å, 1.7 µm, 2.1 mm X 100 mm, 3/pk

Product Number: 176000885

Specifications

The ACQUITY BEH C8 sorbent is less retentive compared to the ACQUITY BEH C18 sorbent. A method developer may prefer the speed offered by a less retentive column, while improving peak shape and chromatographic performance. The trifunctionally bonded BEH particles offer the widest usable pH range (1-12), superior low pH stability, and ultra-low column bleed.

ACQUITY UPLC BEH C18 and C8 Columns

The ACQUITY UPLC CSH C18 Column is a universal C18 column choice, suitable for a broad range of compound classes while providing alternate selectivity to BEH C18. Built on the Charged Surface Hybrid [CSH] particle platform, the CSH C18 Column provides exceptional peak shape and increased loading capacity, particularly for basic compounds under low-pH, weak-ionic-strength mobile-phase conditions.

The vast majority of reversed-phase (RP) LC separations take place on columns that contain C18 or C8 bonded stationary phases due to their stability, retentivity and reproducibility. In addition, these hydrophobic ligands provide the desired separation most of the time. ACQUITY UPLC BEH C18 and C8 columns were designed to be the columns of choice for most UPLC separations by providing the widest pH range and efficiencies.

They incorporate trifunctional ligand bonding chemistries which produce superior low pH stability and ultra-low column bleed. T his low pH stability is combined with the high pH stability of the 1.7 μm BEH particle to deliver the widest usable pH operating range. In addition, these new chemistries also utilize new, proprietary endcapping processes which produce outstanding best peak shape for bases. These bonding chemistries and particle synthesis innovations produce the sharpest peaks, highest efficiencies and maximum MS sensitivities.

ACQUITY UPLC BEH Amide Columns

Since 2003, Waters has developed innovative stationary phases  for Hydrophilic Interaction Chromatography [HILIC] to overcome the  challenge of retaining and separating extremely polar compounds. Based on Waters novel ethylene bridged hybrid [BEH] particle technology, New ACQUITY UPLC® BEH Amide columns utilize a chemically stable, trifunctionally-bonded amide phase, enabling a new dimension in stability and versatility for HILIC separations.

Designed to retain polar analytes and metabolites that are too polar to retain by reversed-phase [RP] chromatography, ACQUITY UPLC BEH Amide columns facilitate the use of a wide range of mobile phase pH [2 -11] to facilitate the exceptional retention of polar analytes spanning a wide range in polarity, structural moiety and pKa.

In addition to enhanced retention of polar compounds, ACQUITY UPLC BEH Amide columns provide increased mass spectrometry response, direct compatibility with sample preparation eluates [PPT, LLE and SPE] as well as orthogonal selectivity compared to reversed-phase materials, making HILIC an attractive alternative separation technique.

ACQUITY UPLC columns

ACQUITY UPLC columns are the most technologically advanced LC columns ever created and are the only columns designed, tested and certified to withstand the pressures of UPLC Technology. Based upon the highly efficient 1.7 µm Bridged Ethyl Hybrid (BEH) particles and 1.8 µm High Strength Silica (HSS) particles, these columns are the first and only columns that allow separation scientists to achieve maximum speed, sensitivity and resolution without compromise. The particles and chemistries of the ACQUITY UPLC column family were carefully chosen to produce the ideal combination of ultra-performance efficiencies, wide pH range and complementary selectivities. ACQUITY UPLC BEH columns and ACQUITY UPLC HSS columns are the only columns recommended for use with ACQUITY UPLC Systems.

There is more to creating a UPLC particle than synthesizing a small particle. Many HPLC particles do not possess the mechanical stability and structural integrity to withstand UPLC operating pressures (e.g., 15000 psi/1000 bar). Why is pressure tolerance important? In order to realize the efficiency gains of sub-2 μm particles, the ability to routinely operate at higher linear velocities (e.g., higher flow rates) is required. These higher linear velocities combined with small, sub-2 μm particles result in higher operating backpressures. Waters has created two highly efficient, pressure-tolerant UPLC particles: the 1.7 μm Ethylene Bridged Hybrid (BEH) particle and the 1.8 μm High Strength Silica (HSS) particle.

The first ACQUITY UPLC particle created was the 1.7 μm Ethylene Bridged Hybrid (BEH) particle. This second generation hybrid particle is one of the key enablers behind UPLC technology and is available in five column chemistries: C18, C8, Shield RP18, Phenyl and HILIC. Because this is a hybrid particle, a wider usable pH range (up to pH 1-12) makes method development faster and easier. BEH particles are also available in HPLC particle sizes (2.5, 3.5, 5 and 10 μm) in the XBridge family of HPLC columns, thus allowing seamless transfer between HPLC and UPLC separations.

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