Waters ACQUITY UPLC BEH HILIC Method Transfer Kit, 130A, 1.7 um to 100 mm, 3.5 um, 2/pk - 186004966

Waters™ BEH HILIC 50mm, 1.7 µm to 100mm, 3.5 µm Method Transfer Kit, 2/pk

Product Number: 186004966

Specifications:

Method transfer kits are designed to preserve the integrity of a separation as it is transferred between UPLC and HPLC platforms. Based on the concept of maintaining column length [L] to particle size [dp] ratio [L/dp], these kits provide an ACQUITY UPLC column with an HPLC column of equivalent selectivity and resolving power. Using the ACQUITY UPLC columns calculator, methods can be fully transferred from HPLC to UPLC or from UPLC to HPLC.

ACQUITY UPLC BEH HILIC Columns

Hydrophilic-Interaction Chromatography [HILIC] is a complementary chromatographic technique that can be used to successfully improve the retention of very polar species as well as provide an orthogonal separation mode for mixtures of polar and ionizable compounds. T his is achieved by utilizing an acetonitrile-rich, low-aqueous mobile phase in combination with a polar stationary phase to elute analytes in order of increasing
hydrophilicity [polarity]. The ACQUITY UPLC BEH HILIC Column utilizes an unbonded BEH particle to improve the retention of very polar basic analytes that are difficult to retain by reversed-phase chromatography while demonstrating complementary selectivity to RPLC. Additionally, the BEH HILIC column provides exceptional chemical stability and peak shape compared to silica-based HILIC stationary phases.

ACQUITY BEH HILIC columns contain unbonded BEH substrate and are designed to retain and separate very polar compounds. These unique columns are optimized and tested to produce efficient, reproducible separations under HPLC HILIC conditions. More rugged than silica based HILIC phases, the BEH particle’s chemical stability results in long column lifetimes.

• The ACQUITY UPLC BEH HILIC Column listed here offers a working pH range of 1-9 pH.
• The ACQUITY UPLC BEH HILIC Column listed here is able to take up to 18000 psi (1240 Bar) pressure.

ACQUITY BEH HILIC columns contain unbonded BEH substrates and are designed to retain and separate very polar compounds. These unique columns are optimized and tested to produce efficient, reproducible separations under HPLC HILIC conditions. More rugged than silica-based HILIC phases, the BEH particle’s chemical stability results in long column lifetimes.

The ACQUITY UPLC BEH HILIC Column employs Hydrophilic-Interaction Chromatography (HILIC), a supplementary chromatographic technology for improving the retention of extremely polar species. Additionally, it has an orthogonal separation mode for polar and ionizable chemical mixtures. This is accomplished by eluting analytes in order to increase hydrophilicity using an acetonitrile-rich, low-aqueous mobile phase paired with a polar stationary phase.

Under HPLC HILIC settings, the unique ACQUITY UPLC BEH HILIC Column has been refined and validated to achieve efficient, repeatable separations. Unbonded BEH substrates are used in the lab equipment, which is designed to retain and separate extremely polar molecules. Long column lifetimes are achieved by combining the silica-based HILIC phases with the chemical stability of BEH particles.

Use the ACQUITY UPLC BEH HILIC Column to influence ionizable chemical retention, selectivity, and sensitivity. The packing materials utilized in the ACQUITY UPLC BEH HILIC Column were specifically designed for use with the ACQUITY UPLC System, resulting in remarkable performance. Find different variations of the lab equipment listed here to meet your analytical needs.

To reach unparalleled levels of efficiency, peak symmetry, and chemical stability, use the ACQUITY UPLC BEH HILIC Column. For extremely polar analytes, the analytical column provides extraordinarily low as well as high pH stability. The usage of the ACQUITY UPLC BEH HILIC Column allows the user to use a wide range of mobile phase pH. Furthermore, the analytical column has a high pressure, allowing for exceptional analysis.

Hydrophilic-Interaction Chromatography [HILIC] is a complementary chromatographic technique that can be used to successfully improve the retention of very polar species as well as provide an orthogonal separation mode for mixtures of polar and ionizable compounds. T his is achieved by utilizing an acetonitrile-rich, low-aqueous mobile phase in combination with a polar stationary phase to elute analytes in order of increasing hydrophilicity [polarity]. The ACQUITY UPLC BEH HILIC Column utilizes an unbonded BEH particle to improve the retention of very polar basic analytes that are difficult to retain by reversed-phase chromatography while demonstrating complementary selectivity to RPLC. Additionally, the BEH HILIC column provides exceptional chemical stability and peak shape compared to silica-based HILIC stationary phases.

BEH (Ethylene Bridged Hybrid) Technology

In 1999, Waters launched the XTerra family of HPLC columns featuring first generation hybrid particle technology (HPT). HPT enabled XTerra columns to become one of the most successful column products in the history of Waters. In HPT, the best properties of inorganic (silica) and organic (polymeric) packings are combined to produce a material that has superior mechanical strength, efficiency, high pH stability and peak shape for bases. The first generation methyl hybrid particles of XTerra columns did not possess the mechanical strength or efficiency necessary to fully realize the potential speed, sensitivity and resolution capabilities of UPLC technology. Therefore, a new pressure-tolerant particle needed to be created. A new, second generation hybrid material was developed which utilizes an ethylene bridged hybrid (BEH) structure. Compared to the first generation methyl hybrid particle of XTerra columns, the BEH particle of ACQUITY UPLC BEH columns exhibits improved efficiency, strength and pH range. BEH Technology™ is a key enabler behind the speed, sensitivity and resolution of UPLC separations.

ACQUITY UPLC columns

ACQUITY UPLC columns are the most technologically advanced LC columns ever created and are the only columns designed, tested and certified to withstand the pressures of UPLC Technology. Based upon the highly efficient 1.7 µm Bridged Ethyl Hybrid (BEH) particles and 1.8 µm High Strength Silica (HSS) particles, these columns are the first and only columns that allow separation scientists to achieve maximum speed, sensitivity and resolution without compromise. The particles and chemistries of the ACQUITY UPLC column family were carefully chosen to produce the ideal combination of ultra-performance efficiencies, wide pH range and complementary selectivities. ACQUITY UPLC BEH columns and ACQUITY UPLC HSS columns are the only columns recommended for use with ACQUITY UPLC Systems.

There is more to creating a UPLC particle than synthesizing a small particle. Many HPLC particles do not possess the mechanical stability and structural integrity to withstand UPLC operating pressures (e.g., 15000 psi/1000 bar). Why is pressure tolerance important? In order to realize the efficiency gains of sub-2 μm particles, the ability to routinely operate at higher linear velocities (e.g., higher flow rates) is required. These higher linear velocities combined with small, sub-2 μm particles result in higher operating backpressures. Waters has created two highly efficient, pressure-tolerant UPLC particles: the 1.7 μm Ethylene Bridged Hybrid (BEH) particle and the 1.8 μm High Strength Silica (HSS) particle.

The first ACQUITY UPLC particle created was the 1.7 μm Ethylene Bridged Hybrid (BEH) particle. This second generation hybrid particle is one of the key enablers behind UPLC technology and is available in five column chemistries: C18, C8, Shield RP18, Phenyl and HILIC. Because this is a hybrid particle, a wider usable pH range (up to pH 1-12) makes method development faster and easier. BEH particles are also available in HPLC particle sizes (2.5, 3.5, 5 and 10 μm) in the XBridge family of HPLC columns, thus allowing seamless transfer between HPLC and UPLC separations.

Related Products:

• TruView pH Control LCMS Certified Clear Glass, 12 x 32 mm, Screw Neck Vial, with Cap and preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk - 186005666CV
• ACQUITY UPLC BEH HILIC VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk - 186003980
• 96-well Sample Collection Plate, 700 µL Round well, 25/pk - 186005837