Welcome, Sign In First time visitor? Create a New Account
Your cart is empty

Waters ACQUITY UPLC Protein BEH SEC Guard Column, 450A, 2.5 um, 4.6 mm x 30 mm, 1/pk - 186006850

Price: £592.00


Qty. 
+-
Download Data Sheet

Waters™ ACQUITY UPLC Protein BEH SEC Guard Column, 450 Å, 2.5 µm, 4.6 mm x 30 mm, 1/pk

Product Number: 186006850

Specifications:

Chemistry SEC
Separation Mode SEC/GPC
Particle Substrate Hybrid
pH Range Min 2.5 pH
pH Range Max 8 pH
Temperature Limits 60 C
Maximum Pressure 3000 psi (207 Bar)
Endcapped No
Bonding Technology SEC
Silanol Activity Low
Molecular Weight Range Min 100000
Molecular Weight Range Max 1500000
Particle Shape Spherical
Particle Size 2.5 µm
Endfitting Type Parker-style
Pore Size 450 Å
QC Tested Protein
Format Guard Column
Packing Solvent 20% methanol in water
Surface Area 80
System UPLC
Particle Technology BEH
Technique LC
USP Classification L33
Inner Diameter 4.6 mm
Length 30 mm
Carbon Load 9%
eCord No
UNSPSC 41115709
Application Protein
Brand ACQUITY UPLC
Product Type Guards

The ACQUITY UPLC Protein BEH SEC Guard Column, 450 Å, 2.5 µm, 4.6 mm x 30 mm kit contains an advanced, ultra-low volume guard device (4.6 mm ID x 30 mm) containing BEH450, SEC, 2.5 µm particles and guard connection tubing (0.005” x 1.75”).

BEH (Ethylene Bridged Hybrid) Technology

In 1999, Waters launched the XTerra family of HPLC columns featuring first generation hybrid particle technology (HPT). HPT enabled XTerra columns to become one of the most successful column products in the history of Waters. In HPT, the best properties of inorganic (silica) and organic (polymeric) packings are combined to produce a material that has superior mechanical strength, efficiency, high pH stability and peak shape for bases. The first generation methyl hybrid particles of XTerra columns did not possess the mechanical strength or efficiency necessary to fully realize the potential speed, sensitivity and resolution capabilities of UPLC technology. Therefore, a new pressure-tolerant particle needed to be created. A new, second generation hybrid material was developed which utilizes an ethylene bridged hybrid (BEH) structure. Compared to the first generation methyl hybrid particle of XTerra columns, the BEH particle of ACQUITY UPLC BEH columns exhibits improved efficiency, strength and pH range. BEH Technology™ is a key enabler behind the speed, sensitivity and resolution of UPLC separations.

ACQUITY UPLC columns

ACQUITY UPLC columns are the most technologically advanced LC columns ever created and are the only columns designed, tested and certified to withstand the pressures of UPLC Technology. Based upon the highly efficient 1.7 µm Bridged Ethyl Hybrid (BEH) particles and 1.8 µm High Strength Silica (HSS) particles, these columns are the first and only columns that allow separation scientists to achieve maximum speed, sensitivity and resolution without compromise. The particles and chemistries of the ACQUITY UPLC column family were carefully chosen to produce the ideal combination of ultra-performance efficiencies, wide pH range and complementary selectivities. ACQUITY UPLC BEH columns and ACQUITY UPLC HSS columns are the only columns recommended for use with ACQUITY UPLC Systems.

There is more to creating a UPLC particle than synthesizing a small particle. Many HPLC particles do not possess the mechanical stability and structural integrity to withstand UPLC operating pressures (e.g., 15000 psi/1000 bar). Why is pressure tolerance important? In order to realize the efficiency gains of sub-2 μm particles, the ability to routinely operate at higher linear velocities (e.g., higher flow rates) is required. These higher linear velocities combined with small, sub-2 μm particles result in higher operating backpressures. Waters has created two highly efficient, pressure-tolerant UPLC particles: the 1.7 μm Ethylene Bridged Hybrid (BEH) particle and the 1.8 μm High Strength Silica (HSS) particle.

The first ACQUITY UPLC particle created was the 1.7 μm Ethylene Bridged Hybrid (BEH) particle. This second generation hybrid particle is one of the key enablers behind UPLC technology and is available in five column chemistries: C18, C8, Shield RP18, Phenyl and HILIC. Because this is a hybrid particle, a wider usable pH range (up to pH 1-12) makes method development faster and easier. BEH particles are also available in HPLC particle sizes (2.5, 3.5, 5 and 10 μm) in the XBridge family of HPLC columns, thus allowing seamless transfer between HPLC and UPLC

Related Products

  • 186006842 - BEH450 SEC Protein Standard Mix
  • 176002996 - ACQUITY UPLC Protein SEC Column 450 Å, 2.5 µm, 4.6 mm x 150 mm and BEH450 SEC Protein Standard Mix
  • 176002997 - ACQUITY UPLC Protein SEC Column 450Å, 2.5 µm, 4.6 mm x 300 mm and BEH450 SEC Protein Standard Mix
  • 186006851 - ACQUITY UPLC Protein BEH SEC Column, 450 Å, 2.5 µm, 4.6 mm X 150 mm, 1/pk
  • 186006852 - ACQUITY UPLC Protein BEH SEC Column, 450 Å, 2.5 µm, 4.6 mm x 300 mm, 1/pk
  • 186009315 - wwPTFE Acrodisc Mini Syringe Filter, 0.45 μm 13 mm 100/Pk

  • Model: 186006850
  • Manufactured by: Waters