CERI L-Column C8 Micro 0.1x50mm, 5um Non Coated - 612351 *OBSOLETE*

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Next generation high performance silica-based micro column

L-column Micro is a high performance column for proteome analysis. It combines the low adsorptive packing media with a proprietary column packing technology and a low dead volume column hardware design. It is expected that L-column Micro will make a sensitivity improvement similar to an upgrade to LC/MS/MS performance.

 Column structure

The column tubing is inert fused silica, available in two formats.

 ?PEEK-sleeved type

The outside of the fused silica is sleeved with PEEK resin. The column is easy to handle and to connect to the MS. The end fitting is 1/16 inch stainless steel. PEEK-sleeved L-column Micro is available in 0.075, 0.1, 0.2 and 0.3 mm I.D.

 ?Non-sleeved type

The outside of the fused silica is not sleeved and has no external end fitting. This allows direct coupling to the MS for minimal dead volume The non-sleeved format is available in 0.075 and 0.1 mm I.D.

Trap column

A trap column is necessary for a large injection volume because the flow rate of nano and micro columns are very small. The ideal trap column must show low adsorption, very small dead volume and high retention. Performance was compared between our trap column and one from another manufacturer using an enzyme digest of a protein which is a mixture of low polarity peptides and high polarity peptides. Our trap column retains them fully. There is very little irreversible adsorption to the packing material. There is no loss of theoretical plate number because of the minimal additional dead volume. The cartridge format is economical sample concentration and protection for the L-column Micro.0.075 mmlica

Proteome analysis

A tryptic digest of BSA is analyzed using LC/MS/MS and the sample concentration and cover ratio is determined using an L-column Micro and another brand. A higher cover ratio means that more amino acid sequences are read. L-column Micro shows advantage at all concentration levels, but excels at the lower levels. With extreme end-capping and very high theoretical plate numbers per column, L-column Micro permits identification of many proteins and is the optimum choice for proteome analysis.  

Low adsorption and high efficiency

L-column Micro is able to separate many peptides because of the extremely low adsorption media and the high efficiency of the proprietary packing technology. This is effective for the identification of proteins and the high efficiency of the column enables good quantitative results at trace levels. It is an optimum column for microanalysis, and methods scale-up easily to preparative column dimensions.