Chromolith CapRod RP-18 Endcapped HighResolution 150-0.1 Capillary Column, 150 A, 1 um, 0.1 x 150 mm - 150404

Merck Chromolith CapRod RP-18 Endcapped HighResolution 150-0.1 Capillary Column, 150 Å, 1 μm, 0.1 mm I.D. x 150 mm Length

Product Number: 150404

Analytics and Sample Preparation > Analytical Chromatography > Analytical HPLC > Chromolith® HPLC Columns > Chromolith® CapRod®

This monolithic Trapping Column is a capillary trap that is used to concentrate samples before transfering them to the analytical Chromolith® CapRod column. Typical flow rates are above 1 µL/min.

In contrast to conventional particle-packed columns, Merck’s Chromolith® HPLC columns are based on state-of-the-art monolithic silica technology. Produced from a continuous piece of porous silica using a sol-gel process, Chromolith® rod columns possess a defined bimodal pore structure with macro- and mesopores in the micro- and nanometer range. The high permeability and porosity of the silica skeleton and the resulting low back pressure allow for more flexible flow rates compared to particulate columns. As a result, Chromolith® HPLC columns enable high-throughput analysis without loss of separation efficiency or peak capacity.

Chromolith® HighResolution CapRod capillary columns have significantly increased efficiency and give better resolution of complex samples, such as peptide mixtures. In order to achieve this higher resolution, the macropore of the monolithic silica has been reduced in size. Typical flow rates are in the region 0.1 - 0.5 µL/min.

The revolutionary bimodal pore structure of Chromolith® HPLC columns provides a unique combination of macropores and mesopores.

The mesopores form the fine porous structure (average pore size 13 nm) and create the large uniform surface area on which adsorption takes place, thereby enabling high-performance chromatographic separation. The macropores (average size 2 μm) allow rapid flow of the mobile phase at low pressure.

The chemical basis of Chromolith® RP-18 endcapped columns – from starting materials to surface modifications – is the same as high-end particulate columns. Thus, their selectivity is comparable to high-quality C18 endcapped reversed-phase packed columns. This allows the use of standard methods when developing new protocols. The columns are based on high-purity silica, hence they minimize the negative effect of trace metals. Furthermore, they are chemically modified with n-alkyl chains that possess a high ligand density, and fully endcapped to reduce the effect of unmodified silanol groups. Chromolith® RP-18 endcapped 2 mm i.d. columns: Ultra-high performance on any instrument Ultra-high performance and extremely low operating pressure make Chromolith® 2 mm columns truly unique. Excellent, ultra-fast results are obtained, not only in the new UHPLC and UPLC® instruments, but equally well in all standard HPLC systems with low dead volume. Chromolith® 2 mm columns have macropores of 1.5 μm in diameter, resulting in a column efficiency that exceeds 100,000 plates/meter. The mesopores are 13 nm (130 A) in diameter, and the surface modification is octadecylsilane with full endcapping.