Merck Chromolith® FastGradient RP-18 Endcapped 50-3 HPLC Column, 130 Å, 2 µm, 3.0 mm I.D. x 50 mm Length
Product Number: 152002
Analytics and Sample Preparation > Analytical Chromatography > Analytical HPLC > Columns for USP Specifications > L1 Octadecylsilane, Chemically Linked to Porous Silica or Ceramic Micro Particles > Chromolith® RP-18 Endcapped > Chromolith® FastGradient RP-18 endcapped 50-3 HPLC column
All Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure. Chromolith® 3mm columns have macropores with 2 µm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilan with full end-capping.
In contrast to conventional particle-packed columns, Merck’s Chromolith® HPLC columns are based on state-of-the-art monolithic silica technology. Produced from a continuous piece of porous silica using a sol-gel process, Chromolith® rod columns possess a defined bimodal pore structure with macro- and mesopores in the micro- and nanometer range. The high permeability and porosity of the silica skeleton and the resulting low back pressure allow for more flexible flow rates compared to particulate columns. As a result, Chromolith® HPLC columns enable high-throughput analysis without loss of separation efficiency or peak capacity.
Chromolith® CapRod is a capillary column that combines the speed of monolithic silica technology with the sensitivity of nano-LC, hence enabling new productivity levels for high-throughput proteomics LC applications. In contrast to microparticulate columns Chromolith® CapRod can be operated at significantly higher flow rates without loss of performance. Separations can be achieved at 1-3 µL/min compared to 200-400 nL/min for microparticulate columns.
The revolutionary bimodal pore structure of Chromolith® HPLC columns provides a unique combination of macropores and mesopores.
The mesopores form the fine porous structure (average pore size 13 nm) and create the large uniform surface area on which adsorption takes place, thereby enabling high-performance chromatographic separation. The macropores (average size 2 μm) allow rapid flow of the mobile phase at low pressure.
Specifications | |
---|---|
Appearance | passes test |
Pressure | ≤ 15 bar |
Theoretical Plates (N/m) (Anthracene) | ≥ 80000 |
Capacity factor (Anthracene) | 3.6 - 4.6 |
Tailing (USP) | 0.90 - 1.30 % |
separation factor (anthracene/progesterone) | 2.30 - 2.60 |
HS Code: | 9027 90 00 |
Quality Level | MQ100 |
Storage class | 10 - 13 Other liquids and solids |
Storage | NoTempLimit |
- Model: 1.52002.0001
- Manufactured by: Merck Chemicals