Chromolith HighResolution RP-18 Endcapped 25-4.6 HPLC Column, 25-4.6, 150 A, 1.15 um, 4.6 x 25 mm - 152020

Merck Chromolith® HighResolution RP-18 Endcapped 25-4.6 HPLC Column, 25-4.6, 150 Å, 1.15 µm, 4.6 mm I.D. x 25 mm Length

Product Number: 152020

Analytics and Sample Preparation > Analytical Chromatography > Analytical HPLC > Chromolith® HPLC Columns > Chromolith® Monolithic HPLC Columns > Chromolith® RP-18 Endcapped Monolithic Silica HPLC Columns > Chromolith® RP-18e HighResolution HPLC Columns > Chromolith® HighResolutionRP-18 endcapped25-4.6 HPLC column

All Chromolith® HighResolution columns are made of monolithic silica with a characteristic bimodal pore structure. Chromolith® 4.6 mm columns have macropores with 1.15 µm diameter, giving a column efficiency exceeding 135,000 plates/meter. The mesopores are 15 nm (150 Å), the surface modification is octadecylsilan with full end-capping.

In contrast to conventional particle-packed columns, Merck’s Chromolith® HPLC columns are based on state-of-the-art monolithic silica technology. Produced from a continuous piece of porous silica using a sol-gel process, Chromolith® rod columns possess a defined bimodal pore structure with macro- and mesopores in the micro- and nanometer range. The high permeability and porosity of the silica skeleton and the resulting low back pressure allow for more flexible flow rates compared to particulate columns. As a result, Chromolith® HPLC columns enable high-throughput analysis without loss of separation efficiency or peak capacity.

Chromolith® CapRod is a capillary column that combines the speed of monolithic silica technology with the sensitivity of nano-LC, hence enabling new productivity levels for high-throughput proteomics LC applications. In contrast to microparticulate columns Chromolith® CapRod can be operated at significantly higher flow rates without loss of performance. Separations can be achieved at 1-3 µL/min compared to 200-400 nL/min for microparticulate columns.

The revolutionary bimodal pore structure of Chromolith® HPLC columns provides a unique combination of macropores and mesopores.

The mesopores form the fine porous structure (average pore size 13 nm) and create the large uniform surface area on which adsorption takes place, thereby enabling high-performance chromatographic separation. The macropores (average size 2 μm) allow rapid flow of the mobile phase at low pressure.