Hydrophilic Interaction Liquid Chromatography (HILIC)
Inertsil HILIC Analytical Columns
HILIC is, abbreviated name for Hydrophilic Interaction Chromatography, and it was developed as an alternative to reversed phase chromatography for highly polar compounds. Inertsil HILIC is the column which chemically bonded with diol group, and it provides excellent peak shape for neutral and basic compounds. In HILIC mode, generally when organic solvent concentration is raised, it shows a strong retention. Moreover, it can get more stable analysis by adding basic solvent to the eluate, such as ammonium acetate.
| Base Material | 3 Series High Purity Silica Gel |
|---|---|
| Particle Size | 3 µm, 5 µm |
| Surface Area | 450 m2/g |
| Pore Size | 100Å (10 nm) |
| Pore Volume | 1.05 mL/g |
| Functional Froup | Diol (Dihydroxypropyl) |
| End-capping | None |
| Carbon Loading | 20.0 % |
| USP Code | L20 |
| pH Range | 2 - 7.5 |
