Amaze TH is tri-modal HILIC and cation- and anion-exchange column designed to separate polar neutral, polar acidic, polar basic, hydrophobic acidic and hydrophobic basic compounds in one run. All functionalities are carried within one ligand which is attached to the surface. The strength of basic and acidic groups can be adjusted by the pH of the mobile phase, allowing to retain compounds even with weak ionizable groups. Highly polar and ionizable groups on the surface allow HILIC separation with lower concentration of ACN, which helps to significantly increase capacity of the column and loadability of compounds during analysis. Retention time for compounds can be adjusted by amount of organic, buffer pH and buffer concentration. Nature of the buffer can be used in some cases to find final tuning for separation of critical pairs of compounds.
