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LiChrospher® 100 RP-18 250-25 Hibar® RT Preparative HPLC Column, 100 Å, 5 µm, 25 × 250 mm - 151483

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LiChrospher® 100 RP-18 Hibar® RT

Inner Diameter (I.D.): 25 mm
Length: 250 mm
Package Contents: 1 column

LiChrospher® 100 RP-18 and LiChrospher® 100 RP-18 endcapped are reliable and versatile traditionally produced 
spherical silica carriers with reversed-phase properties. They are well suited for the chromatography of acidic, 
neutral and weakly basic compounds, substances found frequently in all analytical fields. For LiChrospher® RP-18, 
a masterbatch concept, where several individual batches are used to produce a large batch (“the masterbatch”) 
of the LiChrospher® RP-18 sorbent, is used with the aim of eliminating the variation between the individual batches.

The traditional heart of HPLC is the “ready-to-use” column with threads at both ends onto which reducers for capillary 
connection are screwed. As a rule, precolumns are coupled with the main column via capillaries. If the column is 
exhausted, the user has two possibilities: Refilling, provided aggressive eluents do not prevent this by causing corrosion 
or worn threads, or the whole column is discarded. Column refilling without tube revision is problematic in view
of GLP since each column has a different “history” and a different pretreatment for each new assignment.


Specifications of LiChrospher® 100 RP-18

Sorbent characteristics: 
Particles of silica with octadecyl derivative
Particle shape: spherical
Particle size: 5; 10 μm
Pore size: 100 Å (nm)
Pore volume: 1.25 mL/g
Specific surface area: 350 m2/g
Carbon load: 21.0% C
Coverage of the surface: 3.61 μmol/m2
Efficiency: 55,000 N/m; 20,000 N/m
pH range: pH 2-7.5
Shipping eluent: Acetonitrile/Water


  • Model: 1.51483.0001
  • Manufactured by: Merck Chemicals