Miles Scientific Analtech Silica Gel 150 HL (Hard Layer organic binder) TLC Plates, 250 um, 20 x 20 cm, Channelled, with Preadsorbent Zone, 25/Pk - P66911

Glass-backed Analytical TLC Plates

Miles Scientific Analtech Brand 150 (150Å pore) Silica Gel HL TLC Plates, with Preadsorbent Zone, channelled / channeled - Glass-backed - 250µm thickness - 20 x 20 cm - 25 plates per box 

Product Number: P66911

Weight: 11.10 LBS

HL - Hard Layer organic binder

These plates use a 150 Angstrom pore silica gel.  Its polarity is slightly less than the standard 60 Angstrom pore TLC plates.  It may be necessary to reduce the polarity of the mobile phase to obtain equivalent separation.  However, this decrease produces greater separation in the higher Rf regions of the plate while maintaining resolution in the lower zones.  They are suitable for moderately to strongly polar compounds.

The 150A Silica Gel plates use an organic polymer binder which results in a stable, abrasion resistant surface.  The moderately hard surface offers easier handling and simplifies sample application.  It can be written upon with a dull lead pencil.  The 150A Silica Gel plates are compatible with most organic solvents and will tolerate approximately 70-80% water in the mobile phase.  They are compatible with most visualization reagents but are not recommended for aggressive techniques such as sulfuric acid charring.  Layers are available with and without a UV254 fluorescent indicator.  A variety of sizes is available.

Analtech Brand Silica Gel HL / HLF TLC Plates

• Silica Pore: 150Å
• Silica Particle Size: 8-10 μm average
• Binder: Proprietary polymer (organic)
• Hardness: 200-400 (easy to write on with pencil)

Acceptable Solvents:

All typical chromatography solvents (80% water max)

Visualization Techniques:

Compatible with most visualization reagents except sulfuricn acid charring. Reagent may be dipped or sprayed. Some incompatibility with certain acidic reagents.

General Description:

Best layer choice unless contra-indicated by noted incompatibilities of visualization reagents or mobile phase composition. Hard layer organic binder system is the most rugged, providing easy handling of layer during sample application, mobile phase development, visualization and data interpretation. Rugged layer surface provides consistent results independent of operator technique. Quick development times and uniform visualization properties provide lot-to-lot consistency and reduce seasonal variability in chromatography.

Hard Layer Silica Gel HL & HLF UNIPLATES™ (Organic Binder)

These are the most rugged silica gel plates available. They not only ease handling and sample application, but also permit the use of up to 80% water in the developing solvent without loss of adherence of the adsorbent layer to the glass plate. Organic binder plates are recommended for all TLC applications except those that use vigorous charring for visualization. Virtually all other visualization methods are free from interference from the organic binder.

Hard Layer Silica Gel UNIPLATES represent an advancement in thin layer adsorbent technology. Special high performance silica gel with small average diameter and narrow particle size distribution results in separations 25% to 30% faster than with most other TLC plates. Separated spots are more compact and better resolved, which allows visualization at lower sample levels. The enhanced abrasion resistance means easier handling and sample application because plate surface gouging is virtually eliminated. Analtech Hard Layer UNIPLATES are available with either organic or inorganic binders. Binder choice has little effect on the adsorptive properties of the adsorbent layer.

Preadsorbent UNIPLATES™

• 3cm wide sample application zone
• Concentrates sample spots on the plate
• Simplifies sample preparation and application
• Channeled version available

Preadsorbent UNIPLATES have an inert spotting zone along the bottom edge of the plate. This sample application region serves as a “holding zone” for sample spots until development of the plate is initiated. There is no significant resolution of sample components by the stationary phase in this region. All soluble components migrate with the solvent front, concentrating diffuse sample spots into vertically narrow bands.

The adsorbent zone extends from the upper edge of the preadsorbent zone and covers the remaining area of the TLC plate. This zone exhibits the selective retention of sample components characteristic of the adsorbent employed. Separation of sample components occurs in the adsorbent zone in the same way as on a standard TLC plate.

Preconcentration of the sample results in compact zones at the beginning of the separation. Also, resolution and sensitivity are less dependent on sample application technique since all samples are concentrated to narrow bands regardless of the size or position in the zone of the sample spots.

Channeled UNIPLATES™

Channeled UNIPLATES are available in a variety of adsorbents and plate sizes including the preadsorbent format. These plates have 9 mm wide adsorbent tracks which are separated by 1 mm wide channels where the adsorbent layer has been removed. Channeled plates prevent cross contamination and the spreading of the sample components during development.