Miles Scientific Analtech Unibond Silica Gel HPTLC-HLF (Hard Layer organic binder, Fluorescent indicator) HPTLC UNIPLATES, 150 um, 10 x 20 cm (low form), with Preadsorbent Zone, 50/Pk - P61027-2

Glass-backed Analytical HPTLC Plates

Miles Scientific Analtech Brand HPTLC-HLF TLC Plates, 150µm, 10x20cm (low form), with Preadsorbent Zone, 50Pk

25 Plates /Box, 250 μm Thickness , 15μm particle , 60Å pore

Product Number: P61027-2

Weight: 11.10 LBS

• HL - Hard Layer organic binder
• F - Fluorescent indicator UV254. (Can be added to any adsorbent.)
• HPTLC - High Performance Thin Layer Chromatography

Also available in packs of 25 - P61027

Hard Layer Silica Gel GHL & GHLF UNIPLATES™ (Inorganic Binder)

• Layers tolerate 100% water.
• Contains no organic materials.
• Suitable for aggressive visualization procedures.
• Standard scoring for 20 x 20 cm plates yields four 5 x 20 cm plates.
• Channeled 20 x 20 cm plates have 19 channels (see channeled adapter for TLC Autospotter
• One box of scored 10 x 20 cm plates yields two hundred 2.5 x 10 cm plates

These plates are 100% H20 resistant and are compatible with aqueous developing solvents. They are less rugged than the plates with organic binder but still provide improved handling and sample application compared to Silica Gel G UNIPLATES and are tough enough to write on with a soft pencil, if required.

Hard Layer UNIPLATES with inorganic binder are recommended for all applications including those where visualization occurs through strong charring procedures or in any case in which reagent interaction with an organic binder occurs.

Preadsorbent UNIPLATES™

• 3cm wide sample application zone
• Concentrates sample spots on the plate
• Simplifies sample preparation and application
• Channeled version available

Preadsorbent UNIPLATES have an inert spotting zone along the bottom edge of the plate. This sample application region serves as a “holding zone” for sample spots until development of the plate is initiated. There is no significant resolution of sample components by the stationary phase in this region. All soluble components migrate with the solvent front, concentrating diffuse sample spots into vertically narrow bands.

The adsorbent zone extends from the upper edge of the preadsorbent zone and covers the remaining area of the TLC plate. This zone exhibits the selective retention of sample components characteristic of the adsorbent employed. Separation of sample components occurs in the adsorbent zone in the same way as on a standard TLC plate.

Preconcentration of the sample results in compact zones at the beginning of the separation. Also, resolution and sensitivity are less dependent on sample application technique since all samples are concentrated to narrow bands regardless of the size or position in the zone of the sample spots.