Miles Scientific Analtech Unibond Silica Gel HPTLC-RPS (Hydrocarbon Impregnated, Reversed Phase), HPTLC UNIPLATES, 150 um, 10 x 10 cm, Scored (5 x 5 cm), 25/Pk - P54377

Glass-backed Analytical HPTLC Plates

Miles Scientific Analtech Brand HPTLC-RPS TLC Plates, 150µm, 10x10cm, scored (5x5cm), 25Pk

Product Number: P54377

Weight: 3.10 LBS

• RPS - Hydrocarbon Impregnated (Silica Gel) Reversed Phase Separation
• HPTLC - High Performance Thin Layer Chromatography

Hydrocarbon Impregnated

• Less expensive than bonded phase TLC plates
• Aqueous compatibility for polar samples
• Inorganic binder
• Rapid, inexpensive methods development for HPLC
• Expanded flexibility to a wide range of applications

The RPS impregnated UNIPLATE approximates the adsorption characteristics of the bonded C18 modified silica gels. Therefore, RPS Reversed Phase UNIPLATES are an excellent medium for rapid, inexpensive screening of chromatographic conditions in HPLC method development. Mixtures of water or buffers plus a polar organic modifier (eg., Methanol, acetonitrile, THF, etc.) are easily scouted with RPS UNIPLATES. However, since the hydrocarbon is adsorbed but not chemically anchored to the silica gel, the RPS UNIPLATES cannot tolerate nonpolar organic solvents. Ethyl Acetate or less polar organic solvents will strip the hydrocarbon from the silica gel support.

Reversed Phase Unibond™ Series HPTLC UNIPLATES™

The availability of both normal phase and Reversed Phase HPTLC UNIPLATES™ completes HPTLC as a sensible, efficient and cost effective alternative technique for quantitative analysis. These Reversed Phase adsorbents share the features and benefits of silica gel

HPTLC plates: high efficiency adsorbents, 150 μm layer thickness, and smooth surface for densitometry. In the High Performance format, Analtech offers several varieties of supports. A hydrocarbon impregnated layer, similar to the analytical RPS UNIPLATE, offers complete aqueous solvent compatibility Three nonpolar bonded supports in the UNIBOND™ series are available: RP2, RP8, and RP18.

These fully silanized silica gels share complete compatibility with all organic solvents. However, due to the extreme hydrophobic nature of the RP18 adsorbent, aqueous wettability is limited to approximately 60% water in the mobile phase. Wettability is correspondingly higher with the slightly more polar shorter chain RP series plates.

Scored UNIPLATES™

Most UNIPLATES can be supplied with the glass backing scored at specific locations to permit convenient “snapping” into smaller plates. This Analtech feature provides increased versatility and can mean cost savings over plates already cut to smaller sizes.

Different adsorbents, mobile phases, and analytical conditions can be tested quickly and economically. A plate can be spotted, then snapped into several smaller plates for developing in different solvent mixtures to determine the best separation.

A plate can be spotted, developed, then snapped into several smaller plates, and then subjected to different visualization techniques.

Scored UNIPLATES are available in two standard formats:

1. 20 x 20 cm scored UNIPLATES have three score marks 5 cm apart. Each plate can be snapped to produce four 5 x 20 cm plates or any multiple of 5 cm width.
2. 10 x 20 cm scored UNIPLATES have seven score marks 2.5 cm apart. Each can be snapped to produce eight 2.5 x 10 cm plates or any multiple of 2.5 cm width.