Miles Scientific Analtech UNISIL Silica Gel GF (Gypsum, Fluorescent indicator) Prep TLC Plates, Preadsorbent Zone, Glass-backed, 500 um, 20 x 20 cm, 25/Pk - P40012

Glass-backed Prep TLC Plates

Miles Scientific Analtech Brand UNISIL Silica Gel GF Prep TLC Plates with Preadsorbent Zone, (with Fluorescent indicator UV254) - Glass-backed - 500µm thickness - 20x20cm - 25 plates per box - 10μm particle, 60Å pore

Product Number: P40012

Weight: 12.10 LBS

• G - (Gypsum) Calcium Sulfate Hemihydrate binder
• F - Fluorescent indicator UV254. (Can be added to any adsorbent.)

UNISI™ Silica Gel G & GF UNIPLATES™

These plates are prepared using a special high purity silica gel with a calcium sulfate binder. UNISIL UNIPLATES offer exceptional discrimination for aflatoxins compared to other adsorbent layers.

Analtech Brand Silica Gel G / GF TLC Plates

• Silica Pore: 60Å
• Silica Particle Size: 8-10 μm average
• Binder: Calcium sulfate a.k.a. gypsum (inorganic)
• Hardness: 20-40

Acceptable Solvents:

All typical chromatography solvents (20% water max)

Visualization Techniques:

Compatible with essentially all visualization reagents, even sulfuric acid charring. Spraying reagent is suggested; layer is incompatible with aqueous dipping technique.

General Description:

Considered to be the original “Classic” TLC plate. Gypsum (calcium sulfate) binder produces softer layer for easy scraping and removal of spots or bands for further analysis. This is the best choice when preparative work is needed using thicker coatings to isolate larger quantity of material. Because the binder is inorganic, it is compatible with aggressive visualization techniques such as sulfuric acid charring. Limited water tolerance due to slight solubility of calcium sulfate in water precludes dipping procedure but is applicable with spray techniques.

Silica Gel G & GF UNIPLATES™

Silica Gel G & GF UNIPLATES are the most widely used silica gel TLC plates. No organic substances are added to these products, thus making them compatible with sulfuric acid charring visualization. These plates are commonly scraped from the glass backing to isolate separated components for other analytical procedures. They are compatible with all organic solvents, however, they are not recommended for use in systems containing greater than 20% water because of them solubility of the gypsum binder in aqueous solutions.

The “G” stands for Gypsum and indicates that calcium sulfate hemihydrate (CaSO4•1/2H2O), a refined form of gypsum, has been included in the adsorbent formulation. Gypsum functions as a binder, improving the cohesion of the adsorbent particles and increasing the adhesion of the layer to the glass substrate. The “F” indicates the presence of a short wave UV indicator that is fluorescent at 254 nm.

Preadsorbent UNIPLATES™

• 3cm wide sample application zone
• Concentrates sample spots on the plate
• Simplifies sample preparation and application
• Channeled version available

Preadsorbent UNIPLATES have an inert spotting zone along the bottom edge of the plate. This sample application region serves as a “holding zone” for sample spots until development of the plate is initiated. There is no significant resolution of sample components by the stationary phase in this region. All soluble components migrate with the solvent front, concentrating diffuse sample spots into vertically narrow bands.

The adsorbent zone extends from the upper edge of the preadsorbent zone and covers the remaining area of the TLC plate. This zone exhibits the selective retention of sample components characteristic of the adsorbent employed. Separation of sample components occurs in the adsorbent zone in the same way as on a standard TLC plate.

Preconcentration of the sample results in compact zones at the beginning of the separation. Also, resolution and sensitivity are less dependent on sample application technique since all samples are concentrated to narrow bands regardless of the size or position in the zone of the sample spots.

Preparative TLC plates

Preparative TLC plates have adsorbent layers thicker than the 250 micron layers normally used for analytical separations. Thus, preparative plates can be used for separation of larger quantities of materials. The loading capacity for a given degree of sample component resolution increases roughly as the square root of the increased adsorbent layer thickness. In preparative TLC, materials to be separated are often applied as long streaks rather than spots. After development, specific compounds may be recovered by scraping the adsorbent layers from the plate in the region of interest and eluting the separated material from the adsorbent using a strong solvent. 

• Thicker layers for higher capacity
• Full range of plate sizes ·Prep-scored option for convenient zone location 
• Available with preadsorbent sample loading zone
• Calcium sulfate binder-soft later for easy scraping