Miles Scientific Analtech Silica Gel GF (Gypsum, Fluorescent indicator) with 0.02 M Sodium Acetate TLC Plates, Glass-backed, 250 um, 20 x 20 cm, 25/Pk - spec-009F

Glass-backed Special TLC

Miles Scientific Analtech Silica Gel GF with 0.02 M Sodium Acetate (with Fluorescent indicator UV254) Thin Layer Chromatography (TLC) Plates - Glass-backed - 250 um - 20x20cm - 25/Pk

Product Number: spec-009F

• G - (Gypsum) Calcium Sulfate Hemihydrate binder
• F - Fluorescent indicator UV254. (Can be added to any adsorbent.)
• Fluorescent indicator has green fluorescence at 254 nm

Silica Gel G & GF UNIPLATES™

Silica Gel G & GF UNIPLATES are the most widely used silica gel TLC plates. No organic substances are added to these products, thus making them compatible with sulfuric acid charring visualization.

These plates are commonly scraped from the glass backing to isolate separated components for other analytical procedures. They are compatible with all organic solvents, however, they are not recommended for use in systems containing greater than 20% water because of the solubility of the gypsum binder in aqueous solutions (For water tolerance see the GHL & GHLF series of plates).

The “G” stands for Gypsum and indicates that calcium sulfate hemihydrate (CaSO4•1/2H2O), a refined form of gypsum, has been included in the adsorbent formulation. Gypsum functions as a binder, improving the cohesion of the adsorbent particles and increasing the adhesion of the layer to the glass substrate. The “F” indicates the presence of a short wave UV indicator that is fluorescent at 254 nm. 

Preparative TLC plates have adsorbent layers thicker than the 250 microns. Thus, preparative plates can be used for separation of larger quantities of materials. The loading capacity for a given degree of sample component resolution increases roughly as the square root of the adsorbent layer thickness. In preparative TLC, materials to be separated are often applied as long streaks rather than spots. After development, specific compounds may be recovered by scraping the adsorbent layers from the plate in the region of interest and eluting the separated material from the adsorbent using a strong solvent.