Applications of TLC
TLC is generally used for one of three purposes.
- QUALITATIVE Analysis To determine the presence or absence of a particular substance in a mixture. A rough estimation of the level of the substance may also be performed.
- QUANTITATIVE Analysis To determine, precisely and accurately, the amount of a particular substance in a sample mix.
- PREPARATIVE Analysis To purify and isolate a particular substance by separating it from any contaminants.
All three cases share the common procedures of sample application, chromatographic separation, and sample component visualization. The conditions under which these procedures are performed vary according to the required end result.