COSMOGEL® GST-Accept
Chromatography media for purification of GST-tagged proteins
Nacalai Tesque COSMOGEL GST-Accept Glutathione S-transferase SP Reagent, 5 mL, 1/Pk
Product Number: 09277-56
The GST (glutathione S-transferase) tag is an epitope tag that is used frequently along with the His tag in affinity purification of proteins. Since GST specifically binds to its ligand, glutathione, GST-tagged proteins can be efficiently purified by linking glutathione to a support.
- Extremely durable
- Excellent cost performance
COSMOGEL IEX Series are available in amphoteric ion-exchange type in which two kinds of packing materials are mixed, as well as in widely used anion-exchange type and cation-exchange type.
Ion Exchange Mode | Anion-exchange Type | Cation-exchange Type | Amphoteric ion-exchange Type | |||
Packing Material | Type Q | Type Q-N | Type S | Type S-N | Type M | Type M-N |
Gel / Average Particle Size | Hydrophilic Polymer / 5 µm | |||||
Average Pore Size | 1000 Å | Non-porous | 1000 Å | Non-porous | 1000 Å | Non-porous |
Functional Group | -CH3N+(CH3)3 | -(CH2)3SO3 | -CH3N+(CH3)3 + -(CH2)3SO3- | |||
Protein Binding Capacity | 110-150 mg | 12-20 mg | 70-100 mg | 10-18 mg | 55-75 mg(BSA)/ml 35-50 mg(IgG)/ml | 6-10 mg(BSA)/ml 5-9 mg(IgG)/ml |
BSA/ml-resin | human IgG/ml-resin | |||||
Target Sample | Acidic Proteins / DNA | Basic Proteins | All Proteins | |||
Application | For Purification | For Ultra-fast Analysis For Precise Analysis | For Purification | For Ultra-fast Analysis For Precise Analysis | For Purification | For Precise Analysis |
Column Size I.D. x Length(mm) | 4.6 x 50 | 4.6 x 30 4.6 x 100 | 4.6 x 50 | 4.6 x 30 4.6 x 100 | 4.6 x 50 | 4.6 x 100 |
Product Specifications:
Product description | Cosmogel® GST-Accept |
---|---|
Amount | 100 ml |
Quantity | 1 pc |
Manufacturer | Nacalai Tesque |
Brand | Cosmogel |
Product Type | Bulk Media |
Chemistry Producers Term | GST-Accept |
Ligand | Glutathione |
Particle size | 50 to 150 µm |
matrix | 4% Agarose |
Collection Capacity | >5 mg/mL (recovery amount/gel amount) |
Recommended Linear Velocity | 26 cm/h |
Limit Pressure | 2.6 psi (0.18 bar) |
Supply Status | 50vol% suspension (20vol% ethanol aqueous solution) |
Additional Column Sizes are available - Please contact our Sales Team with your requirements.
Cosmosil Columns Online Catalogue:
- Model: 09277-56
- Manufactured by: Nacalai Tesque