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Cosmosil® RNA Series
Nacalai Tesque COSMOSIL RNA-SEC-2000 Semi-preparative HPLC Guard Column, 5µm, 2000Å, 7.5mm I.D. x 50mm Length, 1/Pk
Product Number: 21096-91
HPLC columns for RNA and Oligonucleotide Separation
RNA-based therapeutics represent a powerful new tool for gene therapy. In RNA production, impurities such as nucleotide triphosphates, abortive transcripts, and DNA templates can affect downstream applications by triggering immune responses. Gel-based methods of purification, such as preparative denaturing polyacrylamide gel electrophoresis (PAGE) and agarose gels, are limited by poor resolution of RNA size and potential chemical modifications from reagents such as formaldehyde. To date, high performance liquid chromatography (HPLC) remains a staple method for purification of messenger RNA (mRNA) and RNA oligonucleotides. Presented here are two HPLC methods: a size exclusion-based method for the purification of long mRNA, and a reversed-phase method for the purification of oligonucleotides.
- Detects impurities not observable with electrophoresis
- Scale up to preparative separations
- Separate long RNA strands from 100 to 5,000 bases
- Two modes available: reversed-phase and size-exclusion
| Packing Material | RNA-RP1 | RNA-SEC-1000 | RNA-SEC-2000 |
|---|---|---|---|
| Separation mode | Reversed-phase | Size-exclusion | |
| Silica gel | Fully-porous high-purity spherical silica gel | ||
| Average particle size (µm) | 5 µm | ||
| Average pore size (nm, approx.) | — | 100 (1,000 Å) | 200 (2,000 Å) |
| Bonded phase | Octadecyl group | Hydrophilic group | |
| Suitable pH range | 2 – 7.5 | ||
| Maximum pressure (MPa) | 15 | ||
Column Specifications:
| Product description | Cosmosil® RNA-SEC-2000 Guard Column 7.5mmx50mm |
|---|---|
| Quantity | 1 pc |
| Manufacturer | Nacalai Tesque |
| Brand | Cosmosil |
| Product Type | SEC/GPC Guard Columns |
| Length | 50 mm |
| Internal Diameter | 7.5 mm |
| Chemistry Producers Term | RNA-SEC-2000 |
| Pore Size | 2000 Å |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| pH Minimum | 2.0 |
| pH Maximum | 7.5 |
| Mode of Separation | Aqueous Size Exclusion Chromatography (SEC) |
| Technology | High-Performance Liquid Chromatography (HPLC) Size Exclusion Chromatography (SEC) Gel Permeation Chromatography (GPC) |
| Matrix | Silica |
| Particle Morphology | spherical, fully porous |
Additional Column Sizes are available - Please contact our Sales Team with your requirements.
Cosmosil Columns Online Catalogue:
- Model: 21096-91
- Manufactured by: Nacalai Tesque
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