COSMOSIL C18-AR-300 C8-AR-300 C4-AR-300 Ph-AR-300
COSMOSIL offers a variety of stationary phases with wide-pore silica gel material for separations of polypeptides and proteins.
Material characteristics
Packing material | 5C18-AR-300 | 5C8-AR-300 | 5C4-AR-300 | 5Ph-AR-300 |
---|---|---|---|---|
Silica gel | high purity porous spherical silica | |||
Average particle size | 5 μm | |||
Average pore size | approx. 300A | |||
Specific surface area | approx. 150 m2/g | |||
Stationary phase | octadecyl group |
octyl group |
butyl group |
phenyl group |
Bonding type | polymeric | |||
Main interactions | hydrophobic interaction | hydrophobic interaction π-π interaction |
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End capping treatment | near-perfect treatment | |||
Carbon content | approx. 12% | approx. 7% | approx. 6% | approx. 7% |
Comparison of separation
5C18-AR-300 | 5C8-AR-300 | 5C4-AR-300 | 5Ph-AR-300 |
---|---|---|---|
Condition | ||||
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Column size | 4.6 mm I.D. x 150 mm | Temperature | 30 oC | |
Mobile phase | A : 0.05%TFA - 20% acetonitrile | Detection | UV 220 nm | |
B : 0.05%TFA - 60% acetonitrile | ||||
B conc. 0 → 100% 20 min linear gradient | ||||
Flow rate | 1.0 ml/min | Sample | Hemoglobine, Bovine 10 μg |
- Model: 34420-63
- Manufactured by: Nacalai Tesque