COSMOSIL C18-AR-300 C8-AR-300 C4-AR-300 Ph-AR-300
COSMOSIL offers a variety of stationary phases with wide-pore silica gel material for separations of polypeptides and proteins.
Material characteristics
| Packing material | 5C18-AR-300 | 5C8-AR-300 | 5C4-AR-300 | 5Ph-AR-300 | 
|---|---|---|---|---|
| Silica gel | high purity porous spherical silica | |||
| Average particle size | 5 μm | |||
| Average pore size | approx. 300A | |||
| Specific surface area | approx. 150 m2/g | |||
| Stationary phase | octadecyl group  | 
			octyl group  | 
			butyl group  | 
			phenyl group  | 
		
| Bonding type | polymeric | |||
| Main interactions | hydrophobic interaction | hydrophobic interaction π-π interaction  | 
		||
| End capping treatment | near-perfect treatment | |||
| Carbon content | approx. 12% | approx. 7% | approx. 6% | approx. 7% | 
Comparison of separation
| 5C18-AR-300 | 5C8-AR-300 | 5C4-AR-300 | 5Ph-AR-300 | 
|---|---|---|---|
| Condition | ||||
|---|---|---|---|---|
| Column size | 4.6 mm I.D. x 150 mm | Temperature | 30 oC | |
| Mobile phase | A : 0.05%TFA - 20% acetonitrile | Detection | UV 220 nm | |
| B : 0.05%TFA - 60% acetonitrile | ||||
| B conc. 0 → 100% 20 min linear gradient | ||||
| Flow rate | 1.0 ml/min | Sample | Hemoglobine, Bovine 10 μg | |
- Model: 34420-63
 - Manufactured by: Nacalai Tesque
 

					
											














