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SeQuant® ZIC®-HILIC 150-1 Capillary HPLC Column, 200 Å, 3.5 µm, 1 x 150 mm - 150480

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SeQuant® ZIC®-HILIC
Inner Diameter (I.D.): 1 mm
Length: 150 mm
Pack Contents: 1 Column


Your ideal choice for separation of all types of polar and hydrophilic compounds is SeQuant® ZIC®-HILIC 
and ZIC®-pHILIC HPLC columns. Reproducible retention for compounds that have proved difficult to separate on 
reversed-phase HPLC columns, is ensured by the high performance, zwitterionic stationary phase in these columns. 
Straightforward separation of compounds such as acids and bases, anions and cations, carbohydrates, metabolites,
metal complexes, amino acids, peptides, protein digests and oligonucleotides can therefore be achieved with a 
selectivity complementary to reversed-phase columns. Enhanced LC/MS sensitivity is an additional benefit of using 
these columns.

SeQuant® ZIC®-HILIC and ZIC®-pHILIC HPLC columns are operated in HILIC (Hydrophilic Interaction Liquid 
Chromatography) mode, which means with buffered aqueous eluents containing high content (> 50%) of organic 
solvents such as acetonitrile.

ZIC®-HILIC is silica-based, has highest separation efficiency, and is suitable for most HILIC applications. Columns 
are available in a wide range of formats from capillary to semi-preparative dimensions, and with several different 
particles sizes and pore sizes. ZIC®-pHILIC is polymer-based, has a wider pH stability range (pH 2-12), making 
it the choice for extra difficult separations demanding more extreme eluent conditions.

SeQuant® ZIC®-HILIC benefits
  • Improved separation of hydrophilic and polar compounds
  • Orthogonal selectivity to Reversed Phase
  • Optimal combination with LC/MS
  • Excellent stability
Characteristics

ZIC®-HILIC and ZIC®-pHILIC sorbents have a bonded stationary phase consisting of a highly polar, permanent zwitterionic 
sulfobetaine structure. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes 
the column overall neutral, with weak, but important, ionic interactions. 
The highly hydrophilic sulfobetaine structure is very good at establishing an immobilized water-rich layer within the 
stationary phase – a feature that is fundamentally important for all HILIC separations. Tuning of the selectivity on 
the ZIC®-HILIC and ZIC®-pHILIC column during method development is facilitated by the pH-independent permanent 
zwitterion, ensuring that only the analytes (and not the column) is affected during eluent optimization.

ZIC®-HILIC is for all classes of polar and hydrophilic compounds

With the ZIC®-HILIC column separation of polar and 
hydrophilic compounds is straightforward. The selectivity 
offered by ZIC®-HILIC is suitable for a wide variety of 
molecules containing hydrophilic or ionizable functional 
groups. This includes compounds such as carbohydrates, 
metabolites, acids and bases, organic and inorganic ions, 
metal complexes, amino acids, peptides, protein digests, 
plant and cell extracts, plus much more.

These compounds are normally characterized by a small 
or negative log P value * and have poor retention on 
reversed-phase columns. The ZIC®-HILIC column is 
designed to retain and separate these types of polar and 
hydrophilic molecules with a selectivity that is orthogonal 
to reversed-phase.


Specifications of SeQuant® ZIC®-HILIC 200 Å

Characteristics: Bonded zwitterionic sulfobetaine on high-purity spherical silica particles
Particle size: 3.5 µm
Surface area: 130 m2/g
pH stability: 2-8
Max temp: 70 °C


  • Model: 1.50480.0001
  • Manufactured by: Merck Chemicals

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