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SeQuant® ZIC®-HILIC PEEK 100-2.1 Metal-free HPLC Column, 200 Å, 3.5 µm, 2.1 x 100 mm - 150447

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SeQuant® ZIC®-HILIC 200 Å, 3.5 µm
Inner Diameter (I.D.): 2.1 mm
Length: 100 mm
Pack Contents: 1 Column


Your ideal choice for separation of all types of polar and hydrophilic compounds is SeQuant® ZIC®-HILIC and ZIC®-pHILIC HPLC columns. Reproducible retention for compounds that have proved difficult to separate on reversed-phase HPLC columns, is ensured by the high-performance, zwitterionic stationary phase in these columns. Straightforward separation of compounds such as acids and bases, anions and cations, carbohydrates, metabolites, metal complexes, amino acids, peptides, protein digests and oligonucleotides can therefore be achieved with a selectivity complementary to reversed-phase columns. Enhanced LC/MS sensitivity is an additional benefit of using these columns.

SeQuant® ZIC®-HILIC and ZIC®-pHILIC HPLC columns are operated in HILIC (Hydrophilic Interaction Liquid Chromatography) mode, which means with buffered aqueous eluents containing high content (> 50%) of organic solvents such as acetonitrile.

ZIC®-HILIC is silica-based, has highest separation efficiency, and is suitable for most HILIC applications. Columns are available in a wide range of formats from capillary to semi preparative dimensions, and with several different particles sizes and pore sizes. ZIC®-pHILIC is polymer-based, has a wider pH stability range (pH 2-12), making it the choice for extra difficult separations demanding more extreme eluent conditions.

SeQuant® ZIC®-HILIC benefits
  • Improved separation of hydrophilic and polar compounds
  • Orthogonal selectivity to Reversed Phase
  • Optimal combination with LC/MS
  • Excellent stability
Characteristics

ZIC®-HILIC and ZIC®-pHILIC sorbents have a bonded stationary phase consisting of a highly polar, permanent zwitterionic sulfobetaine structure. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the column overall neutral, with weak, but important, ionic interactions.

The highly hydrophilic sulfobetaine structure is very good at establishing an immobilized water-rich layer within the stationary phase – a feature that is fundamentally important for all HILIC separations. Tuning of the selectivity on the ZIC®-HILIC and ZIC®-pHILIC column during method development is facilitated by the pH-independent permanent zwitterion, ensuring that only the analytes (and not the column) is affected during eluent optimization.

ZIC®-HILIC is for all classes of polar and hydrophilic compounds

With the ZIC®-HILIC column separation of polar and hydrophilic compounds is straightforward. The selectivity offered by ZIC®-HILIC is suitable for a wide variety of molecules containing hydrophilic or ionizable functional groups. This includes compounds such as carbohydrates,
metabolites, acids and bases, organic and inorganic ions, metal complexes, amino acids, peptides, protein digests, plant and cell extracts, plus much more.

These compounds are normally characterized by a small or negative log P value * and have poor retention on reversed-phase columns. The ZIC®-HILIC column is designed to retain and separate these types of polar and hydrophilic molecules with a selectivity that is orthogonal to reversed-phase.


Specifications of SeQuant® ZIC®-HILIC and ZIC®-pHILIC


CharacteristicsParticle sizeSurface areapH stabilityMax temp
ZIC®-HILIC 100ÅBonded zwitterionic sulfobetaine on high-purity spherical silica particles 3.5 μm180m2/g2-870°C
ZIC®-HILIC 200ÅBonded zwitterionic sulfobetaine on high-purity spherical silica particles3.5, 5 μm130m2/g2-870°C
ZIC®-pHILICBonded zwitterionic sulfobetaine on polymeric particles5 μm-2-1250°C


  • Model: 1.50447.0001
  • Manufactured by: Merck Chemicals

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