Shimadzu Shim-pack Scepter C18-300 Analytical Column, 5 μm, 300 Å, 4.0 x 100 mm, 1/pk - 227-31203-37

Shim-pack Scepter C18-300

Shimadzu Shim-pack Scepter C18-300 Analytical Column, 5 μm, 300 Å, 4mm x 100mm, 1/pk

Part Number: 227-31203-37

Shim-pack Scepter columns consist of an organosilane hybrid particle for excellent stability and performance. The C18-300 phase has a larger pore size than Scepter C18-120 and is less retentive, which reduces analysis time and is more reproducible for the analysis of compounds that are strongly retained on typical C18 columns. It is recommended for mid- to large-sized molecules (above 5000 MW) to ensure retention and avoid size exclusion effects. It is ideal for LC and LCMS analysis of peptides, proteins, intact monoclonal antibodies and their subunits up to 150 kDa, and is compatible with 100% aqueous mobile phase conditions. USP L1, pore size: 300A, pH range: 1-12, max pressure: 45 MPa/4500 bar/6,500 psi, fully endcapped. A guard column is recommended to prolong the analytical column lifetime. Similar to XBridge Peptide BEH C18.

Hybrid Technology for Versatility and Superior Performance

Broad pH tolerance (pH 1 to 12*) for use with a wide range of method conditions Eight stationary phases and Three column hardware types for extensive sample coverage Scalability from analytical UHPLC to preparative purification applications

Achieve excellent stability and performance over a wide range of LC conditions with Shim-pack Scepter™- the next generation organic silica hydrid-based LC columns. Featuring different ligand functional groups, Shim-pack Scepter columns are effective for method development/scouting, and are suitable for a wide variety of applications and sample types. The three particle sizes (1.9 µm, 3 µm, 5 µm) and wide range of column dimensions make Shim-pack Scepter LC columns fully scalable between UHPLC, HPLC, and preparative LC for seamless method transfer between different laboratory instrumentation.

Fully Porous Hybrid Particle Based Column Series

Excellent stability and performance could be achieved under a wide range of LC conditions with Shim-pack Scepter™ LC columns, which are the next generation organic silica hybrid based columns. With different chemistry characteristics, Shim-pack Scepter columns are effective for method development/scouting with suitability for use in a wide variety of applications.

With different particle sizes (1.9 μm, 3 μm, 5 μm) and different column dimensions, Shim-pack Scepter LC columns are fully scalable between UHPLC, HPLC and preparative LC making method transfer seamless between different laboratory instrumentation.

Shim-pack Scepter C18-300 

Shim-pack Scepter C18-300 is a wide-pore organic silica hybrid reversed phase column recommended for the separation of proteins such as monoclonal antibodies and mid-sized molecules such as oligonucleic acids and peptides that may not be retained on 100-120Å pore size columns due to size-exclusion effects. Pore sizes are optimized for large molecular weight compounds and dispersed uniformly in the column, resulting in symmetrical peak shapes and increased resolution of antibodies and nucleic acids compared to a smaller pore size column.

Shim-pack Scepter Chemistries

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